Abstract
Fourier-transform infrared microspectroscopy (FTIRM) allows analysis of mineral content, mineral crystal maturity and mineral composition at ~10-μ spatial resolution. Previous FTIRM analyses comparing 4-μ thick sections from non-decalcified iliac crest biopsies from women with post-menopausal osteoporosis, as contrasted with iliac crest tissue from individuals without evidence of metabolic bone disease, demonstrated significant differences in average mineral content (decreased in osteoporosis) and mineral crystal size/perfection (increased in osteoporosis). More importantly, these parameters, which vary throughout the tissue in relation to the tissue age in healthy bone, showed no such variation in bone biopsies from patients with osteoporosis. The present study compares the spatial and temporal variation in mineral quantity and properties in trabecular bone in high- and low-turnover osteoporosis. Specifically, six biopsies from women (n=5) and one man with high-turnover osteoporosis (age range 39–77) and four women and two men with low turnover osteoporosis (age range 37–63) were compared to ten “normal” biopsies from three men and seven woman (age range: 27–69). “High turnover” was defined as the presence of increased resorptive surface, higher than normal numbers of osteoclasts and greater than or equal to normal osteoblastic activity. “Low turnover” was defined as lower than normal resorptive surface, decreased osteoclast number and less than normal osteoblastic activity. Comparing variations in FTIR-derived values for each of the parameters measured at the surfaces of the trabecular bone to the maximum value observed in multiple trabeculae from each person, the high-turnover samples showed little change in the mineral: matrix ratio, carbonate: amide I ratio, crystallinity and acid phosphate content. The low-turnover samples also showed little change in these parameters, but in contrast to the high-turnover samples, the low-turnover samples showed a slight increase in these parameters, indicative of retarded, but existent resorption and formation. These data indicate that FTIR microspectroscopy can provide quantitative information on mineral changes in osteoporosis that are consistent with proposed mechanisms of bone loss.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sherman S, Hadley EC (1993) Aging and bone quality: an under explored frontier. Calcif Tissues Int 53:S1
Burstein AH, Zika JM, Heiple KG, Klein L (1975) Contribution of collagen and mineral to the elastic-plastic properties of bone. J Bone Joint Surg 57A:956–961
Martin RB, Ishida J (1989) The relative effects of collagen fiber orientation, porosity, density, and mineralization on bone strength. J Biomech 22:419–426
Currey JD (1984) Effects of differences in mineralization on the mechanical properties of bone. Phil Tran R Soc London B304:509–518
Chatterji S, Wall JC, Jeffrey JW (1981) Age-related changes in the orientation and particle size of the mineral phase in human femoral cortical bone. Calcif Tissue Int .33:567–574
Paschalis EP, Betts F, DiCarlo E, Mendelsohn R, Boskey AL (1997) FTIR microspectroscopic analysis of human iliac crest biopsies from untreated osteoporotic bone. Calcif Tissues Int 61:487–492
Gadeleta SJ, Boskey AL, Paschalis E, Carlson C, Menschik F, Baldini T, Peterson M, Rimnac CM (2000) A physical, chemical, and mechanical study of lumbar vertebrae from normal, ovariectomized, and nandrolone decanoate-treated cynomolgus monkeys (Macaca fascicularis). Bone 27:541–550
Paschalis EP, Boskey AL, Kassem M, Eriksen EF (2003) Effect of hormone replacement therapy on bone quality in early postmenopausal women, J Bone Mineral Res 18:955–959
Ou-Yang H, Paschalis EP, Boskey AL, Mendelsohn R (2002) Chemical structure-based 3D reconstruction of human cortical bone from 2D-IR images. Appl Spectrosc 56:419–422
Paschalis EP, Shane E, Lyritis G, Skarantavos G, Mendelsohn R, Boskey A (2004) Bone fragility and collagen cross-links. J Bone Min Res 19:2000–2004
Aringer M, Vierhapper H, Graninger MT, Bernecker P, Smolen JS, Pietschmann P (2000) Successful treatment of high turnover osteoporosis in a patient with adrenocortical insufficiency. Wien Klin Wochenschr 112:334–337
Wronski TJ, Morey-Holton ER (1987) Skeletal response to simulated weightlessness : a comparison of suspension techniques. Aviat Space Environ Med 58:63–68
Delichasios HK, Lane JM, Rivlin RS (1995) Bone histomorphometry in men with spinal osteoporosis. Calcif Tissue Int 56:359–363
De Leo V, Ditto A, la Marca A, Lanzetta D, Massafra C, Morgante G (2000) Bone mineral density and biochemical markers of bone turnover in peri- and postmenopausal women. Calcif Tissue Int 66:263–267
Coco M, Glicklich D, Faugere MC, Burris L, Bognar I, Durkin P, Tellis V, Greenstein S, Schechner R, Figueroa K, McDonough P, Wang G, Malluche H (2003) Prevention of bone loss in renal transplant recipients: a prospective, randomized trial of intravenous pamidronate. J Am Soc Nephrol 14:2669–2676
Parisien M, Cosman F, Morgan D, Schnitzer M, Liang X, Nieves J, Forese L, Luckey M, Meier D, Shen V, Lindsay R, Dempster DW (1997) Histomorphometric assessment of bone mass, structure, and remodeling: a comparison between healthy black and white premenopausal women. J Bone Miner Res 12:948–957
Aparicio S, Doty SB, Camacho NP, Paschalis EP, Spevak L, Mendelsohn R, Boskey AL (2002) Optimal methods for processing mineralized tissues for Fourier transform infrared microspectroscopy. Calcif Tissue Int 70:422–429
Rey C, Collins B, Goehl T, Dickson IR, Glimcher MJ (1989) The carbonate environment in bone mineral: a resolution-enhanced Fourier transform infrared spectroscopy study. Calcif Tissue Int 45:157–164
Mehl B, Delling G, Schlindwein I, Heilmann P, Voia C, Ziegler R, Nawroth P,Kasperk C (2002) Do markers of bone metabolism reflect the presence of a high- or low-turnover state of bone metabolism? Med Klin (Munich) 97:588–594
Christiansen P (2001) The skeleton in primary hyperparathyroidism: a review focusing on bone remodeling, structure, mass, and fracture. APMIS [Suppl] 102:1–52
Miller LM, Novatt JT, Hamerman D, Carlson CS (2004) Alterations in mineral composition observed in osteoarthritic joints of cynomolgus monkeys. Bone 35:498–506
Xu HH, Eichmiller FC, Barndt PR (2001) Effect of fiber length and volume fraction on the reinforcement of calcium phosphate cement. J Mater Sci Mater Med 12:57–65
Tarnowski CP, Ignelzi MA jr, Morris MD (2002) Mineralization of develo** mouse calvaria as revealed by Raman microspectroscopy. J Bone Miner Res 17:1118–1126
Blank RD, Baldini TH, Kaufman M, Bailey S, Gupta R, Yershov Y, Boskey AL, Coppersmith SN, Demant P, Paschalis EP (2003) Spectroscopically determined collagen Pyr/deH-DHLNL cross-link ratio and crystallinity indices differ markedly in recombinant congenic mice with divergent calculated bone tissue strength. Connect Tissue Res 44:134–142
Acknowledgments
This study was supported by NIH grants AR 041325 and AR046505. This investigation was conducted in a facility constructed with support from Research Facilities Improvement Program Grant CO6-RR12538 from the National Center for Research Resources, NIH. The authors would like to thank the staff of the Pathology Department at the Hospital for Special Surgery for their assistance in obtaining the biopsies.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Boskey, A.L., DiCarlo, E., Paschalis, E. et al. Comparison of mineral quality and quantity in iliac crest biopsies from high- and low-turnover osteoporosis: an FT-IR microspectroscopic investigation. Osteoporos Int 16, 2031–2038 (2005). https://doi.org/10.1007/s00198-005-1992-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00198-005-1992-3