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Construction of an RFLP linkage map for cultivated sunflower

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Abstract

 An RFLP linkage map was constructed for cultivated sunflower Helianthus annuus L., based on 271 loci detected by 232 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the map** population. These genetic markers plus a fertility restoration gene, Rf 1, defined 20 linkage groups, covering 1164 cM of the sunflower genome. Of the 71 loci 202 had codominant genotypic segregation, with the rest showing dominant segregation. Thirty-two of the 232 probes gave multiple locus segregation. There were 39 clusters of tightly linked markers with 0 cM distance among loci. This map has an average marker-to-marker distance of 4.6 cM, with 11 markerless regions exceeding 20 cM.

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Authors and Affiliations

  1. U.S. Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, Fargo, ND 58105, USA, , , , , , US

    C. C. Jan, B. A. Vick & J. F. Miller

  2. Biogenetic Services, Inc, Brookings, SD 57006, USA, , , , , , US

    A. L. Kahler

  3. GAEA, Inc, Brookings, SD 57006, USA, , , , , , US

    E. T. Butler III.

Authors
  1. C. C. Jan
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  2. B. A. Vick
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  3. J. F. Miller
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  4. A. L. Kahler
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  5. E. T. Butler III.
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Received: 17 June 1997 / Accepted: 19 June 1997

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Jan, C., Vick, B., Miller, J. et al. Construction of an RFLP linkage map for cultivated sunflower. Theor Appl Genet 96, 15–22 (1998). https://doi.org/10.1007/s001220050703

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  • DOI: https://doi.org/10.1007/s001220050703

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