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Genetic map** of quantitative trait loci and a major locus for resistance to grey leaf spot in maize

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The genetic basis of GLS resistance was dissected using two DH populations sharing a common resistant parent. A major QTL repeatedly detected in multiple developmental stages and environments was fine mapped in a backcross population.

Abstract

Grey leaf spot (GLS), caused by Cercospora zeae-maydis or Cercospora zeina, is a highly destructive foliar disease worldwide. However, the mechanism of resistance against GLS is not well understood. To study the inheritance of this resistance, we developed two doubled haploid (DH) populations sharing a common resistant parent. The two DH populations were grown in two locations representing the typical maize-growing mountain area in Southwest China for 2 years. GLS disease severity was investigated 2 or 3 times until maturity in the 2 years, and the area under the disease progress curve was calculated. Two high-density linkage maps were constructed by genoty**-by-sequencing. A total of 22 quantitative trait loci (QTLs) were detected for GLS resistance, with most QTLs being repeatedly detected in different stages, locations and years. The confidence intervals of two major QTLs (qGLS_Y2-2 and qGLS_Z2-1) on chromosome 2 from the two DH populations overlapped with each other and were integrated into one consensus QTL (qGLS_YZ2-1). Using highly resistant and highly susceptible plants from a BC3 population, we fine mapped this genetic locus to a genomic region of 2.4 Mb. Using a panel of 255 inbred lines from breeding programmes, we detected associations between markers in the qGLS_YZ2-1 region and GLS resistance. The peak marker (ID-B1) will be very useful for marker-assisted breeding for GLS resistance.

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Funding

This research was supported by the National Key Research and Development Program of China (2016YFD0101205).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Lei Du, Feng Yu, Hao Zhang, Kejun Ma, Wangsen ** Yu

  2. Institute of Environment and Plant Protection, Chinese Academy of Agricultural Sciences, Haikou, 571101, China

    **ng Huang

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  1. Lei Du
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  7. Wangsen **n
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  8. **ng Huang
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  9. Yongzhong Liu
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Contributions

LD, KL and YL designed the study. KL and YL supervised the study. LD, HZ, CY, WX and KM performed the experiments. LD, FY, BW and XH analysed the data. LD and KL prepared the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yongzhong Liu or Kede Liu.

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The experiments were performed in compliance with the current laws of China.

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Du, L., Yu, F., Zhang, H. et al. Genetic map** of quantitative trait loci and a major locus for resistance to grey leaf spot in maize. Theor Appl Genet 133, 2521–2533 (2020). https://doi.org/10.1007/s00122-020-03614-z

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