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Genetics and map** of a novel downy mildew resistance gene, Pl 18 , introgressed from wild Helianthus argophyllus into cultivated sunflower (Helianthus annuus L.)

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Abstract

Key message

A novel downy mildew resistance gene, Pl 18 , was introgressed from wild Helianthus argophyllus into cultivated sunflower and genetically mapped to linkage group 2 of the sunflower genome. The new germplasm, HA-DM1, carrying Pl 18 has been released to the public.

Abstract

Sunflower downy mildew (DM) is considered to be the most destructive foliar disease that has spread to every major sunflower-growing country of the world, except Australia. A new dominant downy mildew resistance gene (Pl 18 ) transferred from wild Helianthus argophyllus (PI 494573) into cultivated sunflower was mapped to linkage group (LG) 2 of the sunflower genome using bulked segregant analysis with 869 simple sequence repeat (SSR) markers. Phenoty** 142 BC1F2:3 families derived from the cross of HA 89 and H. argophyllus confirmed the single gene inheritance of resistance. Since no other Pl gene has been mapped to LG2, this gene was novel and designated as Pl 18. SSR markers CRT214 and ORS203 flanked Pl 18 at a genetic distance of 1.1 and 0.4 cM, respectively. Forty-six single nucleotide polymorphism (SNP) markers that cover the Pl 18 region were surveyed for saturation map** of the region. Six co-segregating SNP markers were 1.2 cM distal to Pl 18 , and another four co-segregating SNP markers were 0.9 cM proximal to Pl 18 . The new BC2F4-derived germplasm, HA-DM1, carrying Pl 18 has been released to the public. This new line is highly resistant to all Plasmopara halstedii races identified in the USA providing breeders with an effective new source of resistance against downy mildew in sunflower. The molecular markers that were developed will be especially useful in marker-assisted selection and pyramiding of Pl resistance genes because of their close proximity to the gene and the availability of high-throughput SNP detection assays.

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Acknowledgments

The authors would like to thank Dr. Loren Rieseberg for providing access to the Sunflower Genome Data Repository. We also thank Drs. Gerald Seiler and Steven Xu for critical review of the manuscript, and Angelia Hogness and Cheryl Huckle for technical assistance. This project was supported by the National Sunflower Association Agreement 12-D02, the USDA-ARS CRIS Project No. 5442-21000-039-00D, and the USDA-AMS Specialty Crop Block Grant Program 12-25-B-1689. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.

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  1. USDA-Agricultural Research Service, Northern Crop Science Laboratory, 1605 Albrecht Blvd. N, Fargo, ND, 58102-2765, USA

    L. L. Qi, M. E. Foley & T. J. Gulya

  2. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    X. W. Cai

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Qi, L.L., Foley, M.E., Cai, X.W. et al. Genetics and map** of a novel downy mildew resistance gene, Pl 18 , introgressed from wild Helianthus argophyllus into cultivated sunflower (Helianthus annuus L.). Theor Appl Genet 129, 741–752 (2016). https://doi.org/10.1007/s00122-015-2662-2

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