Abstract
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We finely map a novel resistance gene ( RpsJS ) to Phytophthora sojae in soybean. RpsJS was mapped in 138.9 kb region, including three NBS-LRR type predicted genes, on chromosome 18.
Abstract
Phytophthora root rot (PRR) caused by Phytophthora sojae (P. sojae) has been reported in most soybean-growing regions throughout the world. Development of PRR resistance varieties is the most economical and environmentally safe method for controlling this disease. Chinese soybean line Nannong 10-1 is resistant to many P. sojae isolates, and shows different reaction types to P. sojae isolates as compared with those with known Rps (Resistance to P. sojae) genes, which suggests that the line may carry novel Rps genes or alleles. A map** population of 231 F2 individuals from the cross of Nannong 10-1 (Resistant, R) and 06-070583 (Susceptible, S) was used to map the Rps gene. The segregation fits a ratio of 3R:1S within F2 plants, indicating that resistance in Nannong 10-1 is controlled by a single dominant gene (designated as RpsJS). The results showed that RpsJS was mapped on soybean chromosome 18 (molecular linkage group G, MLG G) flanked by SSR (simple repeat sequences) markers BARCSOYSSR_18_1859 and SSRG60752K at a distance of 0.9 and 0.4 cm, respectively. Among the 14 genes annotated in this 138.9 kb region between the two markers, three genes (Glyma18g51930, Glyma18g51950 and Glyma18g51960) are the nucleotide-binding site and a leucine-rich repeat (NBS-LRR) type gene, which may be involved in recognizing the presence of pathogens and ultimately conferring resistance. Based on marker-assisted resistance spectrum analyses of RpsJS and the map** results, we inferred that RpsJS was a novel gene or a new allele at the Rps4, Rps5 or Rps6 loci.
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References
Allen GC, Flores-Vergara MA, Krasnyanski S, Kumar S, Thompson WF (2006) A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc 1:2320–2325
Dangl JL, Jones JD (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833
Demirbas A, Rector BG, Lohnes DG, Fioritto RJ, Graef GL, Cregan PB, Shoemaker RC, Specht JE (2001) Simple sequence repeat markers linked to the soybean Rps genes for Phytophthora resistance. Crop Sci 41:1220–1227
Diers BW, Mansur L, Imsande J, Shoemaker RC (1992) Map** Phytophthora resistance loci in soybean with restriction fragmentlength polymorphism markers. Crop Sci 32:377–383
Dorrance AE, Schmitthenner AF (2000) New sources of resistance to Phytophthora sojae in the soybean plant introductions. Plant Dis 84:1303–1308
Dorrance AE, McClure SA, Martin SKS (2003) Effect of partial resistance on phytophthora stem rot incidence and yield of soybean in Ohio. Plant Dis 87:308–312
Gao H, Bhattacharyya MK (2008) The soybean-Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binding-leucine rich repeat-like genes and repetitive sequences. BMC Plant Biol 8:29
Gao H, Narayanan NN, Ellison L, Bhattacharyya MK (2005) Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the Rps1-k locus encode Phytophthora resistance in soybean. Mol Plant Microbe Interact 18:1035–1045
Gordon SG, Martin SKS, Dorrance AE (2006) Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci 46:168–173
Gordon SG, Kowitwanich K, Pipatpongpinyo W, St Martin SK, Dorrance AE (2007) Molecular Marker Analysis of Soybean Plant Introductions with Resistance to Phytophthora sojae. Phytopathology 97:113–118
Grant D, Nelson RT, Cannon SB, Shoemaker RC (2010) SoyBase, the USDA-ARS soybean genetics and genomics database. Nucleic Acids Res 38:D843–D846
Han YP, Teng WL, Yu KF, Poysa V, Anderson T, Qiu LJ, Lightfoot DA, Li WB (2008) Map** QTL tolerance to Phytophthora root rot in soybean using microsatellite and RAPD/SCAR derived markers. Euphytica 162:231–239
Li Q, Wan JM (2005) SSRHunter: development of a local searching software for SSR sites. Yi Chuan 27:808–810
Li XP, Han YP, Teng WL, Zhang SZ, Yu KF, Poysa V, Anderson T, Ding JJ, Li WB (2010) Pyramided QTL underlying tolerance to Phytophthora root rot in mega-environments from soybean cultivars ‘Conrad’ and ‘Hefeng 25’. Theor Appl Genet 121:651–658
Lin F, Zhao M, ** J, Johnson A, Zhang B, Abney TS, Hughes TJ, Ma J (2013) Molecular map** of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B. Theor Appl Genet 126:2177–2185
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Sandhu D, Gao HY, Cianzio S, Bhattacharyya MK (2004) Deletion of a disease resistance nucleotide-binding-site leucine-rich-repeat-like sequence is associated with the loss of the Phytophthora resistance gene Rps4 in soybean. Genetics 168:2157–2167
Sandhu D, Schallock KG, Rivera-Velez N, Lundeen P, Cianzio S, Bhattacharyya MK (2005) Soybean phytophthora resistance gene Rps8 maps closely to the Rps3 region. J Hered 96:536–541
Schmitthenner AF (1985) Problems and progress in control of phytophthora root-rot of soybean. Plant Dis 69:362–368
Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183
Song QJ, Jia GF, Zhu YL, Grant D, Nelson RT, Hwang EY, Hyten DL, Cregan PB (2010) Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR_1.0) in soybean. Crop Sci 50:1950–1960
Sugimoto T, Yoshida S, Kaga A, Hajika M, Watanabe K, Aino M, Tatsuda K, Yamamoto R, Matoh T, Walker DR, Biggs AR, Ishimoto M (2011) Genetic analysis and identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge. Euphytica 182:133–145
Sugimoto T, Kato M, Yoshida S, Matsumoto I, Kobayashi T, Kaga A, Hajika M, Yamamoto R, Watanabe K, Aino M, Matoh T, Walker DR, Biggs AR, Ishimoto M (2012) Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans. Breed Sci 61:511–522
Sun S, Zhao JM, Wu XL, Guo N, Wang YC, Gai JY, **ng H (2008) Resistance of soybean germplasm to Phytophthora in Huanhuai Valley. Soybean Sci 27:465–470 (In Chinese,with English abstract)
Sun S, Wu XL, Zhao JM, Wang YC, Tang QH, Yu DY, Gai JY, ** of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed 130:139–143
Van Ooijen JW (2006) JoinMap 4 Software for the calculation of genetic linkage maps in experimental populations. Wageningen, Kyazma B.V
Wang HH, Waller L, Tripathy S, St Martin SK, Zhou LC, Krampis K, Tucker DM, Mao YC, Hoeschele I, Maroof MAS, Tyler BM, Dorrance AE (2010) Analysis of genes underlying soybean quantitative trait loci conferring partial resistance to Phytophthora sojae. Plant Genome 3:23–40
Weng C, Yu K, Anderson TR, Poysa V (2001) Map** genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7. J Hered 92:442–446
Weng CR, Yu KF, Anderson TR, Poysa V (2007) A quantitative trait locus influencing tolerance to Phytophthora root rot in the soybean cultivar ‘Conrad’. Euphytica 158:81–86
Wrather JA (2013) Soybean disease loss estimates for the United States, 1996–2010. http://aes.missouri.edu/delta/research/soyloss.stm. Accessed 5 Nov 2013
Wrather JA, Koenning SR (2009) Effects of diseases on soybean yields in the United States 1996 to 2007. Plant Health Prog doi:10.1094/PHP-2009-0401-01-RS. http://www.plantmanagementnetwork.org/pub/php/research/2009/yields/. Accessed 5 Nov 2013
Wu XL, Zhao JM, Sun S, Yang F, Wang YC, Gai JY, **ng H (2010) A survey of soybean germplasm for resistance to Phytophthora sojae. Euphytica 176:261–268
Wu XL, Zhang BQ, Sun S, Zhao JM, Yang F, Guo N, Gai JY, ** of resistance to Phytophthora sojae of Pm28 in soybean. Agric Sci China 10:1506–1511
Zhang J, ** of the novel Phytophthora resistance gene in a Chinese soybean cultivar. Theor Appl Genet 126:1555–1561
Acknowledgments
We thank Prof. Yuanchao Wang (Nan**g Agricultural University) for providing the 15 isolates of P. sojae. This work was supported by Special Fund for Agro-scientific Research in the Public Interest (201303018), Modern Agro-industry Technology Research System of China (CARS-004-PS10), Jiangsu Provincial Natural Science Foundation (BK2011637) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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The authors declare that they have no conflict of interest.
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Communicated by D. A. Lightfoot.
J. Sun and L. Li contributed equally to this study.
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Sun, J., Li, L., Zhao, J. et al. Genetic analysis and fine map** of RpsJS, a novel resistance gene to Phytophthora sojae in soybean [Glycine max (L.) Merr.]. Theor Appl Genet 127, 913–919 (2014). https://doi.org/10.1007/s00122-014-2266-2
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DOI: https://doi.org/10.1007/s00122-014-2266-2