Abstract
Key message
This manuscript describes the chromosomal location of a new source of stripe rust resistance in wheat. DNA markers closely linked with the resistance locus were identified and validated.
Abstract
A wheat landrace, AUS27858, from the Watkins collection showed high levels of resistance against Australian pathotypes of Puccinia striiformis f. sp. tritici. It was reported to carry two genes for stripe rust resistance, tentatively named YrAW1 and YrAW2. One hundred seeds of an F3 line (HSB#5515; YrAW1yrAW1) that showed monogenic segregation for stripe rust response were sown and harvested individually to generate monogenically segregating population (MSP) #5515. Stripe rust response variation in MSP#5515 conformed to segregation at a single locus. Bulked segregant analysis using high-throughput DArT markers placed YrAW1 in chromosome 4AL. MSP#5515 was advanced to F6 and phenotyped for detailed map**. Novel wheat genomic resources including chromosome-specific sequence and genome zipper were employed to develop markers specific for the long arm of chromosome 4A. These markers were used for further saturation of the YrAW1 carrying region. YrAW1 was delimited by 3.7 cM between markers owm45F3R3 and sun104. Since there was no other stripe rust resistance gene located in chromosome 4AL, YrAW1 was formally named Yr51. Reference stock for Yr51 was lodged at the Australian Winter Cereal Collection, Tamworth, Australia and it was accessioned as AUS91456. Marker sun104 was genotyped on a set of Australian and Indian wheat cultivars and was shown to lack the resistance-linked sun104-225 bp allele. Marker sun104 is currently being used for marker-assisted backcrossing of Yr51 in Australian and Indian wheat backgrounds.
Similar content being viewed by others
References
Akbari M, Wenzl P, Caig V, Carling J, **a L, Yang S, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420
Bariana HS (2003) Breeding for disease resistance. In: Thomas B, Murphy DJ, Murray BG (eds) Encyclopedia of applied plant sciences. Harcourt, Academic Press, pp 244–253
Bariana HS, McIntosh RA (1993) Cytogenetic studies in wheat XV. Location of rust resistance genes in VPM1 and their genetic linkage with other resistance genes in chromosome 2A. Genome 36:476–482
Berkman PJ, Skarshewski A, Manoli S, Lorenc MT, Stiller J, Smits L, Lai K, Campbell E, Kubaláková M, Simková H, Batley J, Doležel J, Hernandez P, Edwards D (2012) Sequencing wheat chromosome arm 7BS delimits the 7BS/4AL translocation and reveals homoeologous gene conservation. Theor Appl Genet 124:423–432
Devos K, Dubcovsky J, Dvořák J, Chinoy CN, Gale MD (1995) Structural evolution of wheat chromosomes 4A, 5A and 7B and its impact on recombination. Theor Appl Genet 91:282–288
Distelfeld A, Uauy C, Olmos S, Schlatter AR, Dubcovsky J, Fahima T (2004) Microcolinearity between a 2-cM region encompassing the grain protein content locus Gpc-6B1 on wheat chromosome 6B and a 350-kb region on rice chromosome 2. Funct Integr Genomics 4:59–66
Feuillet C, Langridge P, Waugh R (2008) Cereal breeding takes a walk on the wild side. Trends Genet 24:24–32
Hernandez P, Martis M, Dorado G, Pfeifer M, Galvez S, Schaaf S, Jouve N, Simkova H, Valarik M, Dolezel J, Mayer KFX (2012) Next-generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content. Plant J 69:377–386
Jaccoud D, Peng K, Feinstein D, Kilian A (2001) Diversity arrays: a solid state technology for sequence information independent genoty**. Nucleic Acids Res 29:e25
Jakobson I, Reis D, Tiidema A, Peusha H, Timofejeva L, Valárik M, Kladivová M, Šimková H, Doležel J, Järve K (2012) Fine map**, phenotypic characterization and validation of non-race-specific resistance to powdery mildew in a wheat-Triticum militinae introgression line. Theor Appl Genet 125:609–623
Kosambi DD (1944) The estimation of map distances from recombination values. Annu Eugen 12:172–175
Manly KF, Cudmore RH, Meer JM (2001) Map Manager QTX, cross platform software for genetic map**. Mamm Genome 12:930–932
McIntosh RA, Dubcovsky J, Rogers J, Morris C, Appels R, **a X (2011) Catalogue of gene symbols for wheat: 2011 supplement
Miftahudin RK, Ma XF, Mahmoud AA, Layton J, Milla MA, Chikmawati T, Ramalingam J, Feril O, Pathan MS, Momirovic GS, Kim S, Chema K, Fang P, Haule L, Struxness H, Birkes J, Yaghoubian C, Skinner R, McAllister J, Nguyen V, Qi LL, Echalier B, Gill BS, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvorák J, Dilbirligi M, Gill KS, Peng JH, Lapitan NL, Bermudez-Kandianis CE, Sorrells ME, Hossain KG, Kalavacharla V, Kianian SF, Lazo GR, Chao S, Anderson OD, Gonzalez-Hernandez J, Conley EJ, Anderson JA, Choi DW, Fenton RD, Close TJ, McGuire PE, Qualset CO, Nguyen HT, Gustafson JP (2004) Analysis of expressed sequence tag loci on wheat chromosome group 4. Genetics 168:651–663
Naranjo T, Roca P, Goicoechea PG, Giraldez (1987) Arm homoeology of wheat and rye chromosomes. Genome 29:873–882
Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107:1235–1242
Poland JA, Brown PJ, Sorrells ME, Jannink JL (2012) Development of high-density genetic maps for barley and wheat using a novel two-enzyme genoty**-by-sequencing approach. PLoS One 7:e32253. doi:10.1371/journal.pone.0032253
Somers D, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25
Valárik M, Linkiewicz AM, Dubcovsky J (2006) A microcolinearity study at the earliness per se gene Eps-A m1 region reveals an ancient duplication that preceded the wheat-rice divergence. Theor Appl Genet 112:945–957
Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78
Wicker T, Mayer KFX, Gundlach H, Mihaela M, Burkhard S, Uwe S, Simkova H, Kubalakova M, Choulet F, Taudien S, Platzer M, Feuillet C, Fahima T, Budak H, Dolezel Y, Keller B, Stein N (2011) Frequent genetic movement and pseudogene evolution is common to the large and complex genomes of wheat, barley, and their relatives. Plant Cell 23:1706–1718
Xue S, Zhang Z, Lin F, Kong Z, Cao Y, Li C, Yi H, Mei M, Zhu H, Wu J, Xu H, Zhao D, Tian D, Zhang C, Ma Z (2008) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181–189
Yan L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, SanMiguel P, Bennetzen JL, Echenique V, Dubcovsky J (2004) The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science 303:1640–1644
Acknowledgments
The first author thanks the Australian Centre for International Agricultural Research (ACIAR) for the award of John Allwright Fellowship to pursue Ph.D. study. We acknowledge financial support from Grant Agency of Czech Republic (project GACR P501/10/1740), Internal Grant Agency PrF-2012-001 and the Grains Research Development Corporation (GRDC) Australia. We are thankful to Diversity Arrays Technology Pty Ltd, Australia for sharing sequence information of clones.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by M. Xu.
Rights and permissions
About this article
Cite this article
Randhawa, M., Bansal, U., Valárik, M. et al. Molecular map** of stripe rust resistance gene Yr51 in chromosome 4AL of wheat. Theor Appl Genet 127, 317–324 (2014). https://doi.org/10.1007/s00122-013-2220-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-013-2220-8