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Identification and molecular map** of two QTLs with major effects for resistance to Fusarium head blight in wheat

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Abstract

Fusarium head blight (FHB) is a devastating disease of wheat worldwide. Novel sources of resistance are critical for improving FHB resistance levels in wheat. From a large-scale evaluation of germplasm for reactions to FHB, we identified one wheat accession (PI 277012) that consistently showed a high level of resistance in both greenhouse and field experiments. To characterize the FHB resistance in this accession, we developed a doubled haploid (DH) map** population consisting of 130 lines from the cross between PI 277012 and the hard red spring wheat cultivar ‘Grandin’. The DH population was then evaluated for reactions to FHB in three greenhouse seasons and five field environments. Based on a linkage map that consisted of 340 SSR markers spanning 2,703 cM of genetic distance, two major quantitative trait loci (QTLs) for FHB resistance were identified on chromosome arms 5AS and 5AL, with each explaining up to 20 and 32% of the variation in FHB severity, respectively. The two QTLs also showed major effects on reducing the percentage of Fusarium damaged kernels (FDK) and deoxynivalenol (DON) accumulation in seeds. FHB resistance has not previously been reported to be associated with this particular genomic region of chromosome arm 5AL, thus indicating the novelty of FHB resistance in PI 277012. Plant maturity was not associated with FHB resistance and the effects of plant height on FHB resistance were minor. Therefore, these results suggest that PI 277012 is an excellent source for improving FHB resistance in wheat. The markers identified in this research are being used for marker-assisted introgression of the QTLs into adapted durum and hard red spring wheat cultivars.

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Acknowledgments

The authors wish to thank Daryl Klindworth, Qijun Zhang, Shaukat Ali, Guotai Yu, Qun Sun, Joseph Mullins, Sarah Underdahl, and Stan Stancyk for assistance with field disease evaluations, Danielle Holmes for preparing inoculum in greenhouse disease evaluations, and Mary Osenga for technical support in molecular marker analysis. This material is based upon work supported by the US Department of Agriculture, under Agreement No. 0506-XU-103 and the CRIS Project No. 5442-22000-080-033-00D. This is a cooperative project with the US Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

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  1. Chenggen Chu

    Present address: Heartland Plant Innovations Inc., 217 Southwind Place, Manhattan, KS, 66503, USA

Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    Chenggen Chu & Shaobin Zhong

  2. USDA-ARS, Northern Crop Science Laboratory, 1605 Albrecht Blvd. North, Fargo, ND, 58102-2765, USA

    Zhixia Niu, Shiaoman Chao, Timothy L. Friesen, Justin D. Faris & Steven S. Xu

  3. Langdon Research Extension Center, North Dakota State University, Langdon, ND, 58249, USA

    Scott Halley

  4. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Elias M. Elias

  5. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA

    Yanhong Dong

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  1. Chenggen Chu
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Correspondence to Steven S. Xu.

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Chu, C., Niu, Z., Zhong, S. et al. Identification and molecular map** of two QTLs with major effects for resistance to Fusarium head blight in wheat. Theor Appl Genet 123, 1107–1119 (2011). https://doi.org/10.1007/s00122-011-1652-2

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