Abstract
Somatic hybridization between bread wheat and tall wheatgrass (Agropyron elongatum) has generated fertile introgression progenies with novel combinations of high molecular weight glutenin subunits (HMW-GS). Most of these novel HMW-GS alleles were stably inherited. Sixteen HMW-GS sequences were PCR amplified from three introgression progeny lines and sequenced. The alignment of these sequences indicated that five, probably derived from point mutations of the parental genes, whereas eight likely represent the product of replication slippage. Three Glu-1Ay sequences appear to have lost the transposon presented in the parental gene. Two subunits carry an additional cysteine residue, which may be favorable to the quality of end-use product. We demonstrate that novel HMW-GS alleles can be rapidly generated via asymmetric somatic hybridization.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00122-009-0973-x/MediaObjects/122_2009_973_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00122-009-0973-x/MediaObjects/122_2009_973_Fig2_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00122-009-0973-x/MediaObjects/122_2009_973_Fig3_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00122-009-0973-x/MediaObjects/122_2009_973_Fig4_HTML.gif)
Similar content being viewed by others
References
Anderson OD, Greene FC (1989) The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid wheat. Theor Appl Genet 77:689–700
Brettell RI, Dennis ES (1991) Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern. Mol Gen Genet 229:365–372
Feng DS, **a GM, Zhao SY, Chen FG (2004) Two quality-associated HMW glutenin subunits in a somatic hybrid line between Triticum aestivum and Agropyron elongatum. Theor Appl Genet 110:136–144
Forde J, Malpica J-M, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Res 13:6817–6832
Gupta RB, MacRitchie F (1994) Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1, of common wheats. II. Biochemical basis of the allelic effects on dough properties. J Cereal Sci 19:19–29
Harberd NP, Bartels D, Thompson RD (1986) DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem Genet 24:579–596
Harberd NP, Flavell RB, Thompson RD (1987) Identification of a transposon-like insertion in a Glu-1 allele of wheat. Mol Gen Genet 209:326–332
Hirochika H, Sugimoto K, Otsuki Y, Tsugawa H, Kanda M (1996) Retrotransposons of rice involved in mutations induced by tissue culture. Proc Natl Acad Sci USA 93:7783–7788
Kumar S, Tamura K, Nei M (2004) MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformatics 5:150–163
Lafiandra D, D’Ovidio R, Porceddu E, Margiotta B, Colaprico G (1993) New data supporting high Mr glutenin subunit 5 as the determinant of quality differences among the pairs 5 + 10 vs 2 + 12. J Cereal Sci 18:197–205
Lafiandra D, Tucci GF, Pavoni A, Yurchetta T, Margiott B (1997) PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat. Theor Appl Genet 94:235–240
Liu H, Shi L, Zhao JS, **a GM (2006) Genetic characteristic of high molecular weight glutenin subunits in somatic hybrid wheat lines—potential application to wheat breeding. J Agric Food Chem 54:5007–5013
Lorincz MC, Groudine M (2001) CmC(ayt)GG methylation: a new epigenetic mark in mammalian DNA? Proc Natl Acad Sci USA 98:10034–10036
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Wells RD (1996) Molecular basis of genetic instability of triplet repeats. J Biol Chem 271:2875–2878
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Shewry PR, Tatham AS (1990) The prolamin storage proteins of cereal seeds: structure and evolution. Biochem J 267:1–12
Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003a) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nut Res 45:219–302
Shewry PR, Halford NG, Lafiandra D (2003b) Genetics of wheat gluten proteins. Adv Genet 49:111–184
**a GM, **ang FN, Zhou AF, Wang H, Chen HM (2003) Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet 107:299–305
Acknowledgments
This research was supported by the National Basic Research 973 Program of China, No. 2009CB118300, National Natural Science Foundation of China No. 30871320 and National 863 High Technology Research and Development Project 2006AA100102.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by X. **a.
Heng Liu and Shuwei Liu have contributed equally to the work.
Rights and permissions
About this article
Cite this article
Liu, H., Liu, S. & **a, G. Generation of high frequency of novel alleles of the high molecular weight glutenin in somatic hybridization between bread wheat and tall wheatgrass. Theor Appl Genet 118, 1193–1198 (2009). https://doi.org/10.1007/s00122-009-0973-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-009-0973-x