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Map** of genes associated with leptine content of tetraploid potato

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Abstract

High content of leptine glycoalkaloids present in Solanum chacoense has been associated with genetic resistance to Colorado potato beetle (Leptinotarsa decemlineata [Say]). From an unrecorded accession of S. chacoense, the North Dakota State University breeding program has developed a tetraploid genotype, ND4382-19, that contains foliar leptines. In this study, using a segregating population, ND5873 (ND4382-19 × Chipeta), and GC-MS to analyze foliar content of alkaloids, two loci, involved in the synthesis of leptines were identified. They segregated as two complementary epistatic genes that allowed the synthesis of leptinidine (Lep) and acetyl-leptinidine (AL), respectively. Partial AFLP maps for both parents were developed using 97 individuals from population ND5873. The total lengths mapped for ND4382-19 and Chipeta were 1,883 and 1,021 cM, respectively. The marker for Lep was located at the distal end of simplex-coupling linkage group R37. Expansion of the initial map** population and analysis of Lep-containing individuals allowed us to identify the linkage group (R35) that enabled synthesis of AL. By the use of simple sequence repeat markers, linkage group R37 (Lep) and linkage group R35 (AL) have been identified as homologs of chromosomes II and VIII, respectively.

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References

  • Boiteau G (1988) Control of the Colorado potato beetle, Leptinotarsa decemlineata (Say). Learning from the Soviet experience. Bull Entomol Soc Can 20:9–14

    Google Scholar 

  • Bonierbale MR, Plaisted RL, Pineda O, Tanksley SD (1994) QTL analysis of trichomes-mediated resistance in potato. Theor Appl Genet 87:973–987

    Article  CAS  Google Scholar 

  • Bradshaw JE, Hackett CA, McNicol JW, Meyer RC, Milbourne D, Phillips MS, Waugh R (1998) Identification of AFLP and SSR markers associated with quantitative resistance to Globodera pallida (Stone) in tetraploid potato (Solanum tuberosum subsp tuberosum) with a view to marker-assisted selection. Theor Appl Genet 97:202–210

    Article  Google Scholar 

  • Bradshaw JE, Pande B, Bryan GJ, Hackett CA, Mclean K, Stewart HE (2004) Interval map** of quantitative trait loci for resistance to late blight (Phytophthora infestans (Mont.) de Bary), height and maturity in a tetraploid population of potato (Solanum tuberosum subsp. tuberosum). Genetics 168:983–995

    Article  PubMed  CAS  Google Scholar 

  • Deahl KL, Sinden SL (1987) A technique for rapid detection of leptine glycoalkaloids in potato foliage. Am Potato J 64:285–290

    Google Scholar 

  • Ferro DN, Logan JA, Voss RH, Elkinton JS (1985) Colorado potato beetle (Coleoptera: Chrysomelidae) temperature-dependent growth and feeding rates. Environ Entomol 14:343–348

    Google Scholar 

  • Forgash AJ (1985) Insecticide resistance in the Colorado potato beetle. Res Bull Mass Agric Exp Stn 704:33–52

    Google Scholar 

  • Friedman M, McDonald GM (1997) Potato glycoalkaloids: chemistry, analysis, safety, and plant physiology. Crit Rev Plant Sci 16:55–132

    CAS  Google Scholar 

  • Fulton TM, Chunwongse J, Tanksley SD (1995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol Biol Rep 13:207–209

    CAS  Google Scholar 

  • Gebhardt C, Valkonen JPT (2001) Organization of genes controlling disease resistance in the potato genome. Ann Rev Phytopath 39:79–102

    Article  CAS  Google Scholar 

  • Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage map** in Solanum tuberosum. Theor Appl Genet 78:65–75

    Article  Google Scholar 

  • Gebhardt C, Mugniery D, Ritter E, Salamini F, Bonnel E (1993) Identification of RFLP markers closely linked to the H1 gene conferring resistance to Globodera rostochiensis in potato. Theor Appl Genet 85:541–544

    Article  CAS  Google Scholar 

  • Hackett CA, Luo ZW (2003) TetraploidMap: construction of linkage map in autotetraploid species. J Hered 94:358–359

    Article  PubMed  CAS  Google Scholar 

  • Hackett CA, Bradshaw JE, Meyer RC, McNicol JW, Milbourne D, Waugh R (1998) Linkage analysis in tetraploid species: a simulation study. Genet Res 71:143–154

    Article  CAS  Google Scholar 

  • Hutvágner G, Bánfalvi Z, Milánkovics I, Silhavy D, Polgár Z, Horváth S, Wolters P, Nap JP (2001) Molecular markers associated with leptinine production are located on chromosome 1 in Solanum chacoense. Theor Appl Genet 102:1065–1071

    Article  Google Scholar 

  • Jacobs JME, van Eck HJ, Arens P, Verkerk-Bakker B, Hekkert BteL, Bastiaanssen HJM, El-Kharbotly A, Pereira A, Jacobsen E, Stiekema WJ (1995) A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers. Theor Appl Genet 91:289–300

    Article  CAS  Google Scholar 

  • Jolivet P (1991) The Colorado beetle menaces Asia (Leptinotarsa decemlineata Say) (Coleoptera: Chrysomelidae). L’Entomologiste 47:29–48

    Google Scholar 

  • Kuhn R, Löw I (1961) Zur Konstitution der Leptine. Chem Ber 94:1088–1095

    CAS  Google Scholar 

  • Lawson DR, Erb WA, Miller AR (1992) Analysis of solanum alkaloids using internal standardization and capillary gas chromatography. J Agric Food Chem 40:2186–2191

    Article  CAS  Google Scholar 

  • Lawson DR, Veilleux RE, Miller AR (1993) Biochemistry and genetics of Solanum chacoense steroidal alkaloids: natural resistance factors to the Colorado potato beetle. Curr Top Bot Res 1:335–352

    Google Scholar 

  • Liu BH (1998) Statistical genomics: linkage, map** and QTL analysis. CRC Press, Boca Raton

    Google Scholar 

  • Lorenzen JH, Balbyshev NF (1997) ND2858-1: a useful source of resistance to the Colorado potato beetle. Am Potato J 74:331–335

    Google Scholar 

  • Lorenzen JH, Balbyshev NF, Lafta AM, Casper H, Tian X, Sagredo B (2001) Resistant potato selections contain leptine and inhibit development of the Colorado potato beetle (Coleoptera: Chrysomelidae). J Econ Entomol 94:1260–1267

    PubMed  CAS  Google Scholar 

  • Luo ZW, Hackett CA, Bradshaw JE, McNicol JW, Milbourne D (2001) Construction of a genetic linkage map in tetraploid species using molecular markers. Genetics 157:1369–1385

    PubMed  CAS  Google Scholar 

  • Medina TB, Fogelman E, Chani E, Miller AR, Levin I, Levy D, Veilleu RE (2002) Identification of molecular markers associated with leptine in reciprocal backcross families of diploid potato. Theor Appl Genet 105:1010–1018

    Article  PubMed  CAS  Google Scholar 

  • Meyer RC, Milbourne D, Hackett CA, Bradshaw JE, McNicol JW, Waugh R (1998) Linkage analysis in tetraploid potato and associations of markers with quantitative resistance to late blight (Phytophthora infestans). Mol Gen Genet 259:150–160

    Article  PubMed  CAS  Google Scholar 

  • Milbourne D, Meyer RC, Collins AJ, Ramsay LD, Gebhardt C, Waugh R (1998) Isolation, characterization and map** of simple sequence repeat loci in potato. Mol Gen Genet 259:233–245

    Article  PubMed  CAS  Google Scholar 

  • Osman SF, Sinden SL, Deahl KL, Moreau R (1987) Metabolism of solanidine by microsomal fractions from Solanum chacoense. Phytochem 26:3163–3165

    Article  CAS  Google Scholar 

  • Ott J (1992) Strategies for characterization of highly polymorphic markers in human gene map**. Am J Hum Genet 51:283–290

    PubMed  CAS  Google Scholar 

  • Ragsdale D, Radcliffe EB (1999) VegEdge: potatoes-Colorado potato beetle. Minnesota Extension Service, University of Minnesota. http://www.extension.umn.edu/vegipm/vegpest/cpb.htm

  • Rahardja V, Whalon ME (1995) Inheritance of resistance to Bacillus thurgensis subsp tenebrosis CryIIIA delta-endotoxin in Colorado potato beetle (Coleoptera: Chrysomelidae). J Econ Entomol 88:21–26

    PubMed  CAS  Google Scholar 

  • Ronning CM, Sanford LL, Kobayashi RS, Kowalski SP (1998) Foliar leptine production in segregating F1, inter-F1, and backcross families of Solanum chacoense Bitter. Am J Potato Res 75:137–143

    CAS  Google Scholar 

  • Ronning CM, Stommel JR, Kowalski SP, Sanford LL, Kobayashi RS, Pineda O (1999) Identification of molecular markers associated with leptine production in a population of Solanum chacoense Bitter. Theor Appl Genet 98:39–46

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Sanford LL, Kobayashi RS, Deahl KL, Sinden SL (1996) Segregation of leptines and other glycoalkaloids in Solanum tuberosum (4×) × Solanum chacoense (4×) crosses. Am Potato J 73:21–33

    CAS  Google Scholar 

  • Silhavy D, Szentesi Á, Bánfalvi Z (1996). Solanum chacoense lines with different alkaloid contents—a potential source of genes involved in leptine synthesis. Acta Agro Hung 44:113–120

    CAS  Google Scholar 

  • Sinden SL, Sanford LL, Cantelo WW, Deahl KL (1986a). Leptine glycoalkaloids and resistance to the Colorado potato beetle (Coleoptera: Chrysomelidae) in Solanum chacoense. Environ Entomol 15:1057–1062

    CAS  Google Scholar 

  • Sinden SL, Cantelo WW, Sanford LL, Deahl KL (1986b) Segregation of leptine glycoalkaloid in Solanum chacoense Bitter. J Agric Food Chem 34:372–377

    Article  CAS  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Stürckow B, Löw I (1961) Effect of several Solanum glycoalkaloids on the Colorado potato beetle (Letinotarsa decemlineata, Say). Entomol Expt Appl 4:133–142

    Article  Google Scholar 

  • Tian X (1998) Application of RFLP subtraction in plant genetic investigation. MS Thesis, North Dakota State University

  • Tingey WM (1984) Glycoalkaloids as pest resistance factors. Am Potato J 61:157–167

    CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    PubMed  CAS  Google Scholar 

  • Wu KK, Burnquist W, Sorrells ME, Tew TL, Moore PH, Tanksley SD (1992) The detection and estimation of linkage in polyploids using single-dose restriction fragments. Theor Appl Genet 83:294–300

    Article  Google Scholar 

  • Yencho GC, Kowalski SP, Kobayashi RS, Sinden SL, Bonierbale MW, Deahl KL (1998) QTL map** of foliar glycoalkaloid aglycones in Solanum tuberosum × S. berthaultii potato progenies quantitative variation and plant secondary metabolism. Theor Appl Genet 97:563–574

    Article  CAS  Google Scholar 

  • Yencho GC, Kowalski SP, Kennedy GG, Sanford LL (2000) Segregation of leptine glycoalkaloids and resistance to Colorado potato beetle (Leptinitarsa decemlineata (Say)) in a F2 Solanum tuberosum (4×) × S. chacoense (4×) potato progenies. Am J Potato Res 77:167–178

    Article  CAS  Google Scholar 

  • Yu KF, Pauls KP (1993) Segregation of random amplified polymorphic DNA markers and strategies for molecular map** in tetraploid alfalfa. Genome 36:844–851

    CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Instituto de Investigaciones Agropecuarias de Chile (INIA), CRI-Remehue, Panamericana km 8, Ruta 5 norte, Casilla 24-O, Osorno, Chile

    B. Sagredo

  2. Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND, 58105, USA

    H. Casper

  3. Department of Plant Science, North Dakota State University, Fargo, ND, 58105, USA

    A. Lafta

  4. Plant Soil and Entomological Sciences Department, University of Idaho, Moscow, ID, 83844, USA

    J. Lorenzen

Authors
  1. B. Sagredo
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  2. A. Lafta
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  3. H. Casper
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  4. J. Lorenzen
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Correspondence to J. Lorenzen.

Additional information

Communicated by G. Wenzel.

H. Casper (deceased)

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Sagredo, B., Lafta, A., Casper, H. et al. Map** of genes associated with leptine content of tetraploid potato. Theor Appl Genet 114, 131–142 (2006). https://doi.org/10.1007/s00122-006-0416-x

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  • DOI: https://doi.org/10.1007/s00122-006-0416-x

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