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Development of EST-derived CAPS and AFLP markers linked to a gene for resistance to ryegrass blast (Pyricularia sp.) in Italian ryegrass (Lolium multiflorum Lam.)

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Abstract

Ryegrass blast, also called gray leaf spot, is caused by the fungus Pyricularia sp. It is one of the most serious diseases of Italian ryegrass (Lolium multiflorum Lam.) in Japan. We analyzed segregation of resistance in an F1 population from a cross between a resistant and a susceptible cultivar. The disease severity distribution in the F1 population suggested that resistance was controlled by a major gene (LmPi1). Analysis of amplified fragment length polymorphisms with bulked segregant analysis identified several markers tightly linked to LmPi1. To identify other markers linked to LmPi1, we used expressed sequence tag-cleaved amplified polymorphic sequence (EST-CAPS) markers mapped in a reference population of Italian ryegrass. Of the 30 EST-CAPS markers screened, one marker, p56, flanking the LmPi1 locus was found. The restriction pattern of p56 amplification showed a unique fragment corresponding to the resistant allele at the LmPi1 locus. A linkage map constructed from the reference population showed that the LmPi1 locus was located in linkage group 5 of Italian ryegrass. Genotype results obtained from resistant and susceptible cultivars indicate that the p56 marker is useful for introduction of the LmPi1 gene into susceptible germplasm in order to develop ryegrass cultivars with enhanced resistance to ryegrass blast.

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Acknowledgements

We would like to thank Y. Sumida, H. Kajihara, and K. Nishimi (Yamaguchi Prefecture Agricultural Experiment Station) for providing ryegrass blast fungus and for suggestions on the inoculation test. We are grateful to S. Sato, K. Suginobu, and S. Yazaki (GAFSA) for their good advice and to E. Kobayashi (GAFSA) for her technical assistance. This study was financially supported by a grant from the Japan Racing Association.

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Author notes

  1. Chenglong Ding

    Present address: Institute of Animal Husbandry Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, **ao Ling Wei, Nan**g, 210014, China

  2. Rie Ozaki

    Present address: Yamaguchi Office of Agriculture and Forestry, 6-10 Kanda-cho, Yamaguchi, 753-0064, Japan

Authors and Affiliations

  1. Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association, 388-5 Higashiakada, Nasushiobara, Tochigi, 329-2742, Japan

    Yuichi Miura, Chenglong Ding, Mariko Hirata, Wataru Takahashi & Hongwei Cai

  2. Yamaguchi Prefecture Agricultural Experiment Station, 1419 Oouchi-mihori, Yamaguchi, 753-0214, Japan

    Rie Ozaki

  3. National Institute of Livestock and Grassland Science, 768 Senbonmatsu, Nasushiobara, Tochigi, 329-2793, Japan

    Masahiro Fujimori & Kazuhiko Mizuno

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  1. Yuichi Miura
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  8. Kazuhiko Mizuno
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Correspondence to Yuichi Miura.

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Communicated by T. Sasaki

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Miura, Y., Ding, C., Ozaki, R. et al. Development of EST-derived CAPS and AFLP markers linked to a gene for resistance to ryegrass blast (Pyricularia sp.) in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 111, 811–818 (2005). https://doi.org/10.1007/s00122-005-0001-8

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