Abstract
Quinoa (Chenopodium quinoa Willd.) is an important seed crop for human consumption in the Andean region of South America. It is the primary staple in areas too arid or saline for the major cereal crops. The objective of this project was to build the first genetic linkage map of quinoa. Selection of the map** population was based on a preliminary genetic similarity analysis of four potential map** parents. Breeding lines ‘Ku-2’ and ‘0654’, a Chilean lowland type and a Peruvian Altiplano type, respectively, showed a low similarity coefficient of 0.31 and were selected to form an F2 map** population. The genetic map is based on 80 F2 individuals from this population and consists of 230 amplified length polymorphism (AFLP), 19 simple-sequence repeat (SSR), and six randomly amplified polymorphic DNA markers. The map spans 1,020 cM and contains 35 linkage groups with an average marker density of 4.0 cM per marker. Clustering of AFLP markers was not observed. Additionally, we report the primer sequences and map locations for 19 SSR markers that will be valuable tools for future quinoa genome analysis. This map provides a key starting point for genetic dissection of agronomically important characteristics of quinoa, including seed saponin content, grain yield, maturity, and resistance to disease, frost, and drought. Current efforts are geared towards the generation of more than 200 mapped SSR markers and the development of several recombinant-inbred map** populations.
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Acknowledgements
This research was supported with grants from the McKnight Foundation, the Holmes Family Foundation, and the Erza Taft Benson Agriculture and Food Institute. We thank Robert Jones, Eric Steward, and David Butler of Brigham Young University for their advice and technical assistance with the AFLP analysis.
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Maughan, P.J., Bonifacio, A., Jellen, E.N. et al. A genetic linkage map of quinoa (Chenopodium quinoa) based on AFLP, RAPD, and SSR markers. Theor Appl Genet 109, 1188–1195 (2004). https://doi.org/10.1007/s00122-004-1730-9
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DOI: https://doi.org/10.1007/s00122-004-1730-9