Abstract
The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F2 population of 1,400 plants (ThibautRdg2a×Mirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.
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Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipmann DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Arru L, Nicks RE, Lindhout P, Valè G, Francia E, Pecchioni N (2002) Genomic regions determining resistance to leaf stripe (Pyrenophora graminea) in barley. Genome 45:460–466
Arru L, Francia E, Pecchioni N (2003a) Isolate-specific QTLs of resistance to leaf stripe (Pyrenophora graminea) in the Steptoe×Morex spring barley cross. Theor Appl Genet 106:668–675
Arru L, Faccini N, Govoni C, Cattivelli L, Pecchioni N, Delogu G, Stanca AM, Valè G (2003b) The PCR-based marker MWG2018 linked to the Rdg2a leaf stripe resistance gene is a useful tool for assessing barley resistance in breeding programs. Crop Sci 43:1036–1042
Ayliffe MA, Collins NC, Ellis JG, Pryor A (2000) The maize rp1rust resistance gene identifies homologues in barley that have been subjected to diversifying selection. Theor Appl Genet 100:1144–1154
Backes G, Madsen LH, Jaiser H, Stougaard J, Herz M, Mohler V, Jahoor A (2003) Localisation of genes for resistance against Blumeria graminis f. sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley ( Hordeum vulgare ssp. spontaneum) line. Theor Appl Genet 106:353–362
Brueggeman R, Druka A, Kudrna D, Kleinhofs A (2001) Efficient cloning of resistance gene analogs from barley. Barley Genet Newsl 31:24–27
Brueggeman R, Rostoks N, Kudrna D, Kilian A, Han F, Chen J, Druka A, Steffenson B, Kleinhofs A (2002) The barley stem rust-resistance gene Rpg1is a novel disease-resistance gene with homology to receptor kinases. Proc Natl Acad Sci USA 99:9328–9333
Collins N, Park R, Spielmeyer W, Ellis J, Pryor AJ (2001) Resistance gene analogs in barley and their relationship to rust resistance genes. Genome 44:375–381
Dangl JL, Jones JDG (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833
Delseny M (2003) Towards an accurate sequence of the rice genome. Curr Opin Plant Biol 6:101–105
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Giese H, Holm-Jensen AG, Jensen HP, Jensen J (1993) Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers. Theor Appl Genet 85:897–900
Goff SA, Ricke D, Lan T-H, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange BM, Moughamer T, **a Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun W-L, Chen L, Cooper B, Park S, Wood TC, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller RM, Bhatnagar S, Adey N, Rubano T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. Japonica). Science 296:92–100
Graner A, Bauer E, Kellermann A, Kirchner S, Muraya JK, Jahoor A, Wenzel G (1994) Progress of RFLP-map construction in winter barley. Barley Genet Newsl 23:53–59
Hammond-Kosack KE, Parker JE (2003) Deciphering plant-pathogen communication: fresh perspecitives for molecular resistance breeding. Curr Opin Biotech 14:177–193
Han F, Kilian A, Chen JP, Kudrna D, Steffenson B, Yamamoto K, Matsumoto T, Sasaki T, Kleinhofs A (1999) Sequence analysis of a rice BAC clone covering the syntenous barley Rpg1 region. Genome 42:1071–1076
Huang L, Gill BS (2001) An RGA-like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat. Theor Appl Genet 103:1007–1013
Hulbert SC, Webb CA, Smith SM, Sun Q (2001) Resistance gene complexes: evolution and utlization. Annu Rev Phytopathol 39:285–312
Kilian A, Kudrna DA, Kleinhofs A, Yano M, Kurata N, Steffenson B, Sasaki T (1995) Rice-barley synteny and its application to saturation map** of the barley Rpg1 region. Nucleic Acids Res 23:2729–2733
Kilian A, Chen J, Han F, Steffenson B, Kleinhofs A (1997) Towards map-based cloning of the barley stem rust resistance genes Rpg1and Rpg4using rice as an intergenomic cloning vehicle. Plant Mol Biol 35:187–195
Künzel G, Korzun L, Meister A (2000) Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on translocation breakpoints. Genetics 154:397–412
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Leister D, Kurth J, Laurie DA, Yano M, Sasaki T, Devos K, Graner A, Schulze-Lefert P (1998) Rapid re-organization of resistance gene homologs in cereal genomes. Proc Natl Acad Sci USA 95:370–375
Leister D, Kurth J, Laurie DA, Yano M, Sasaki T, Graner A, Schulze-Lefert P (1999) RFLP and physical map** of resistance gene homologues in rice (O. sativa) and barley (H. vulgare). Theor Appl Genet 98:509–520
Ling HQ, Zhu Y, Keller B (2003) High resolution map** of the leaf rust disease resistance gene Lr1 in wheat and characterization of BAC clones from the Lr1 locus. Theor Appl Genet 106:875–882
Madsen LH, Collins NC, Rakwalska M, Backes G, Sandal N, Krusell L, Jensen J, Waterman EH, Jahoor A, Ayliffe M, Pryor AJ, Langridge P, Schulze-Lefert P, Stougaard J (2003) Barley disease resistance gene analogs of the NBS-LRR class: identification and map**. Mol Gen Genomics 269:150–161
Neff MM, Neff JD, Chory J, Pepper AE (1998) dCAPS, a simple technique for genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J 14:387–392
Pecchioni N, Faccioli P, Toubia-Rahme H, Valè G, Terzi V (1996) Quantitative resistance to barley leaf stripe (Pyrenophora graminea) is dominated by one major locus. Theor Appl Genet 93:97–101
Porta-Puglia A, Delogu G, Vannacci G (1986) Pyrenophora graminea on winter barley seed: effect on disease incidence and yield losses. J Phytopathol 117:26–33
Rostoks N, Zale JM, Soule J, Brueggeman R, Druka A, Kudrna D, Steffenson B, Kleinhofs A (2002) A barley gene family homologous to the maize rust resistance gene Rp1-D. Theor Appl Genet 104:1298–1306
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Seah S, Sivasithamparam K, Karakousis A, Lagudah ES (1998) Cloning and characterisation of a family of disease resistance gene analogs from wheat and barley. Theor Appl Genet 97:937–945
Siebert PD, Chenchik A, Kellogg DE, Lukyanov A, Lukyanov SA (1995) An improved method for walking in uncloned genomic DNA. Nucleic Acids Res 23:1087–1088
Skou JP, Haahr V (1987) Screening for and inheritance of resistance to barley leaf stripe (Drechslera graminea). Risø report 554, Risø National Laboratory, Roskilde, Denmark
Stein N, Herren G, Keller B (2001) A new DNA extraction method for high-throughput marker analysis in a large-genome species such as Triticum aestivum. Plant Breed 120:354–356
Tacconi G., Cattivelli L, Faccini N, Pecchioni N, Stanca AM, Valè G (2001) Identification and map** of a new leaf stripe resistance gene in barley (Hordeum vulgare L.). Theor Appl Genet 102:1286–1291
The Arabidopsis Genome Initiative (2000) Analysis of the genome of the flowering plant Arabidopsis thaliana. Nature 408:796–815
Thomsen SB, Jensen HP, Jensen J, Skou JP, Jørgensen JH (1997) Localization of a resistance gene and identification of sources of resistance to barley leaf stripe. Plant Breed 116:455–459
Wei F, Gobelman-Werner K, Morroll SM, Kurth J, Mao L, Wing R, Leister D, Schulze-Lefert P, Wise RP (1999) The Mla (Powdery Mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240 kb DNA interval on chromosome 5S (1HS) of barley. Genetics 153:1929–1948
Acknowledgements
The authors thank Nadia Faccini and Donata Pagani for technical assistance. We would like to thank Wolfgang Spielmeyer and Evans Lagudah for the ssCH4 probe and Lene Madsen and Jens Stougaard (University of Aarhus) for the S-9202 DNA sequence. This work was supported by the Italian MiPAF, Project “Protezione delle piante mediante l’uso di marcatori molecolari (PROMAR)” and scheda 7/90 MiPAF. N.C. was funded by Syngenta.
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Bulgarelli, D., Collins, N.C., Tacconi, G. et al. High-resolution genetic map** of the leaf stripe resistance gene Rdg2a in barley. Theor Appl Genet 108, 1401–1408 (2004). https://doi.org/10.1007/s00122-003-1557-9
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DOI: https://doi.org/10.1007/s00122-003-1557-9