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Molecular tagging and genetic map** of the disease resistance gene RppQ to southern corn rust

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Abstract

Southern corn rust (SCR), Puccinia polysora Underw, is a destructive disease in maize (Zea mays L.). Inbred line Qi319 is highly resistant to SCR. Results from the inoculation test and genetic analysis of SCR in five F2 populations and five BC1F1 populations derived from resistant parent Qi319 clearly indicate that the resistance to SCR in Qi319 is controlled by a single dominant resistant gene, which was named RppQ. Simple sequence repeat (SSR) analysis was carried out in an F2 population derived from the cross “Qi319×340”. Twenty SSR primer pairs evenly distributed on chromosome10 were screened at first. Out of them, two primer pairs, phi118 and phi 041, showed linkage with SCR resistance. Based on this result, eight new SSR primer pairs surrounding the region of primers phi118 and phi 041 were selected and further tested regarding their linkage relation with RppQ. Results indicated that SSR markers umc1,318 and umc 2,018 were linked to RppQ with a genetic distance of 4.76 and 14.59 cM, respectively. On the other side of RppQ, beyond SSR markers phi 041 and phi118, another SSR marker umc1,293 was linked to RppQ with a genetic distance of 3.78 cM. Because the five linkage SSR markers (phi118, phi 041, umc1,318, umc 2,018 and umc1,293) are all located on chromosome 10, the RppQ gene should also be located on chromosome 10. In order to fine map the RppQ gene, AFLP (amplified fragment length polymorphism) analysis was carried out. A total 54 AFLP primer combinations were analyzed; one AFLP marker, AF1, from the amplification products of primer combination E-AGC/M-CAA, showed linkage with the RppQ gene in a genetic distance of 3.34 cM. Finally the RppQ gene was mapped on the short arm of chromosome 10 between SSR markers phi 041 and AFLP marker AF1 with a genetic distance of 2.45 and 3.34 cM respectively.

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References

  • Akagi H, Yokozeki Y, Inagaki A, Fujimura T (1996) Microsatallite DNA markers for rice chromosomes. Theor Appl Genet 93:1071–1077

    Article  CAS  Google Scholar 

  • Bailey BA, Schun W, Fred-eriksen RA, Bockholt AJ (1987) Identification of slow-rusting resistance to Puccinia polysora in maize inbreds and single crosses. Plant Dis 71:518–521

    Google Scholar 

  • Chen CX, Yang DE, Yu YJ, Wang ZL, Wang B (2003) Studies on southern corn rust and its resistance. Acta Phytopathol Sin 33:86–87

    Google Scholar 

  • Chin ECL, Senior ML, Smith JSC (1996) Maize simple repetitive DNA sequences: abundance and allele variation. Genome 39:866–873

    CAS  PubMed  Google Scholar 

  • Duan DR, He HZ (1984) Description of a rust Puccinia polysora on corn in Hainan Island. Acta Mycol Sin 3:125–126

    Google Scholar 

  • Futrell MC (1975) Puccinia polysora on maize associated with crop** practice and genetic homogeneity. Phytopathology 65:1041–1042

    Google Scholar 

  • Futrell MC, Hooker AL, Scott GE (1975) Resistance on maize to corn rust, controlled by a single dominant gene. Crop Sci 15:597–599

    Google Scholar 

  • Hillel J, Schapp T, Haberfield A, Jeffreys AJ, Plotzky Y, Cahaner A, Lave U (1990) DNA fingerprints applied to gene introgression in breeding program. Genetics 124:783–789

    CAS  PubMed  Google Scholar 

  • Hoisington DA, Khairallah MM, Gonzales-de-Leon D (1994) Laboratory protocols. CIMMYT Applied Molecular Genetics Laboratory, CIMMYT, Hisfoa, Mexico, DF

  • Holland JB, Uhr DV, Jeffers D, Goodman MM (1998) Inheritance of resistance to southern corn rust in tropical-by-corn-belt maize populations. Theor Appl Genet 96:232–241

    Article  Google Scholar 

  • Hooker AL, Yarwood CE (1966) Culture of Puccinia sorghi on detached leaves of corn and Oxalis corniculata. Phytopathology 56:536–539

    Google Scholar 

  • Hospital F, Chevalet C, Mulsant P (1992) Using markers in gene-introgression breeding programs. Genetics 132:1199–1210

    CAS  PubMed  Google Scholar 

  • Hulbert SH, Bennetzen JL (1991) Recombination at the Rp1 locus of maize. Mol Gen Genet 226:377–382

    CAS  PubMed  Google Scholar 

  • Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Etoh T (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Liu YY, Wang J (1999) Southern corn rust occurred in Hebei Province in 1998. Plant Protect:53

    Google Scholar 

  • Lubberstedt T, DuBle C, Melchinger A (1998) Application of microsatellites from maize to teosinte and other relatives of maize. Plant Breed 117:447–450

    Google Scholar 

  • McMullen MD, Simcox K (1995) Genomic organization of disease- and insect-resistance genes in maize. Mol Plant-Microbe Interact 8:811–815

    Google Scholar 

  • Melching JS (1975) Corn rust: type, races and destructive potential. In: The American Seed Trade Association, pp 90–115

  • Rhind D, Waterston JM, Deighton FC (1952) Occurrence of Puccinia polysora Underw in West Africa. Nature 169:631

    Google Scholar 

  • Rodrigues-Ardon. R, Scott GE, Hennen JF (1980) Maize yield losses caused by southern corn rust. Crop Sci 20:812–814

    Google Scholar 

  • Scott GE, King SB, Armour JW (1984) Inheritance of resistance to southern corn rust in the maize population. Crop Sci 24:265–267

    Google Scholar 

  • Ullstrup AJ (1965) Inheritance and linkage of a gene determining resistance in maize to an American race of Puccinia polysora. Phytopathology 55:425–428

    Google Scholar 

  • Visscher PM, Haley CS, Thompson R (1996) Marker-assisted introgression in backcross breeding programs. Genetics 144:1923-1932

    CAS  PubMed  Google Scholar 

  • Vos PR, Hogers MB (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    CAS  PubMed  Google Scholar 

  • Wang SL (1999) Southern corn rust was epidemic in the Province of Shandong, Henan and Jiangsu. Plant Protect Technol Dissemin 3:40–41

    Google Scholar 

  • Ye JC (2000) The first inbred line Qi319 immune to maize southern rust bred in china. Sci Agricult Sin 33:110

    Google Scholar 

  • Zummo N (1988) Components contributing to partial resistance in maize to Puccinia polysora. Plant Dis 72:157–160

    Google Scholar 

Download references

Acknowledgements

This work was supported by the 10th Five-Year Science and Technology Research Program of China (2001BA511B04-01) and the State High-Tech Program of China (2001AA211111).

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Authors and Affiliations

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Bei**g, China

    C. X. Chen, D. E. Yang, C. J. Ye, D. M. **, M. L. Weng & B. Wang

  2. College of Agronomy, Shandong Agricultural University, 271018, Taian, China

    C. X. Chen, Z. L. Wang & Y. B. Zhao

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  1. C. X. Chen
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  2. Z. L. Wang
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  3. D. E. Yang
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  4. C. J. Ye
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  5. Y. B. Zhao
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  6. D. M. **
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  7. M. L. Weng
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  8. B. Wang
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Correspondence to B. Wang.

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Communicated by H. F. Linskens

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Chen, C.X., Wang, Z.L., Yang, D.E. et al. Molecular tagging and genetic map** of the disease resistance gene RppQ to southern corn rust. Theor Appl Genet 108, 945–950 (2004). https://doi.org/10.1007/s00122-003-1506-7

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  • DOI: https://doi.org/10.1007/s00122-003-1506-7

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