Abstract
A clone, 8380-1, selected fromSolanum chacoense (PI 458310) for its high foliage content of the leptine glycoalkaloids, a factor in resistance to Colorado potato beetle, was doubled in chromosome number from 2n=2x=24 to 2n=4x=48. Three 4x clones were crossed with sixS. tuberosum (4x) clones. Foliage glycoalkaloid contents were measured for 452 F1 hybrids from 15 crosses. The 4xchacoense parental clones were not different in respect to glycoalkaloid contents and were similar to the original 2x clone. All F1 hybrids synthesized foliage leptines ranging from 9 to 369 mg/100 g fresh weight (fw) with a mean content of 113 mg/100 g fw. The proportion of leptines in the total glycoalkaloid content ranged from 1% to 62% with a mean of 25%. The 4xchacoense parent mean leptine content was 1482 mg/ 100 g fw which was 90% of the total glycoalkaloid content. Tubers from 136 hybrids and the threechacoense parental clones were tested for glycoalkaloid contents. The tuber solanine + chaconine contents of the 136 hybrids ranged from 30 to 180 mg/100 g fw with a mean of 79. The mean tuber content of the threechacoense parental clones was 157 mg/100 g fw. Leptines were not found in any of the tubers.
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Literature Cited
Bushway, R.J., J.L. Bureau, and J. King. 1986. Modification of the rapid high-performance liquid Chromatographic method for the determination of potato glycoalkaloids. J Agric Food Chem 34:277–279.
Carman, Jr, A.S., S.S. Kuan, G.M. Ware, O.J. Francis, Jr., and G.P. Kirschenheuter. 1986. Rapid high-performance liquid Chromatographic determination of the potato glycoalkaloids α-solanine and α-chaconine. J Agric Food Chem 34:279–282.
Deahl, K.L., W.W. Cantelo, S.L. Sinden, and L.L. Sanford. 1991. The effect of light intensity on Colorado potato beetle resistance and foliar glycoalkaloid concentration of fourSolanum chacoense clones. Am Potato J 68:659–666.
Deahl, K.L., R.J. Young, and S.L. Sinden. 1973. A study of the relationship of late blight resistance to glycoalkaloid content in fifteen potato clones. Am Potato J 50:248–253.
Friedman, M. and C.E. Levin. 1992. Reversed-phase high-performance liquid Chromatographic separation of potato glycoalkaloids and hydrolysis products on acidíc columns. J Agric Food Chem 40:2157–2163.
Kobayashi, R.S., S.L. Sinden, and L.L. Sanford. 1994. Effect of ploidy on foliar glycoalkaloid concentration and Colorado potato beetle resistance inSolanum chacoense. Am Potato J 71:680. (Abstract).
Kuhn, R. and I. Low. 1961. Zur konstitution der leptine. Chem Ber 94:1088–1095.
Maga, J.A. 1994. Glycoalkaloids inSolanaceae. Food Rev Intnl 10:385–418.
Sanford, L.L. and T.L. Ladd, Jr. 1992. Performance of populations derived by selecting for resistance to potato leafhopper in a 4XSolanum tuberosum × 2XSolanum chacoense cross. Am Potato J 69:391–400.
Anon. SAS/STAT Guide, Version 6. 1987. SAS Institute Inc., Cary, N.C.
Sinden, S.L. and L.L. Sanford. 1981. Origin and inheritance of solamarine glycoalkaloids in commercial potato cultivars. Am Potato J 58:305–325.
Sinden, S.L., L.L. Sanford, W.W. Cantelo, and K.L. Deahl. 1986. Leptine glycoalkaloids and resistance to the Colorado potato beetle (Coleoptera:Chrysomelidae) inSolanum chacoense. Environ Entomol 15:1057–1062.
Sinden, S.L., L.L. Sanford, and K.L. Deahl. 1986. Segregation of leptine glycoalkaloid inSolanum chacoense Bitter. J Agric Food Chem 34:372–377.
Sinden, S.L., W.W. Cantelo, L.L. Sanford, and K.L. Deahl. 1991. Allelochemically mediated host resistance to the Colorado potato beetle,Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Mem Ent Soc Can 157:19–28.
Sokal, R.R. and F.J. Rohlf. 1969. Biometry. W.H. Freeman and Co., San Francisco, CA. 776 p.
Struckow, B. and I. Low. 1961. Die Wirkung einiger Solanam-alkaloidglykoside auf den kartoffelkaferLeptinotarsa decemlineata Say. Entomol Expt Appl 4:133–142.
Vogel, H.A., A. Jatisatienr, and R. Bauer. 1990. [Quantitative analysis of the glycoalkaloids solasonine and solamargine and their localization in leaves ofSolanum laciniatum Ait.] Agnew Bot 64:393–400. [In German]
Wolf, M.J. and B.M. Duggar. 1946. Estimation and physiological role of solanine in the potato. J Agric Res 73:1–32.
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Sanford, L.L., Kobayashi, R.S., Deahl, K.L. et al. Segregation of leptines and other glycoalkaloids inSolanum tuberosum (4x) ×S. chacoense (4x) crosses. American Potato Journal 73, 21–33 (1996). https://doi.org/10.1007/BF02849301
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DOI: https://doi.org/10.1007/BF02849301