Abstract
ALactobacillus plantarum of vegetable origin produced a bacteriocin inhibitory toListeria monocytogenes. The antimicrobial agent was inactivated by proteolytic enzymes, was resistant to heat (100°C for 30 min) and stable over a wide pH range (pH 2–10), and displayed a bactericidal mode of action. Growth inhibition ofL. monocytogenes depend on bacteriocin concentration. The antilisterial efficiency depended on the strain ofL. monocytogenes used but was not influenced by the growth phase of this strain. A decrease in absorbance overtime, indicative of cell lysis, was also observed. The significance of the results is discussed in relation to the potential of the bacteriocin in controllingListeria-associated food-borne hazards in foods.
Similar content being viewed by others
References
Atrih A., Michel R.M., Lefebvre G.: Detection of bacteriocins produced byLactobacillus plantarum strains isolated from different foods.Microbios75, 117–123 (1993).
Daeschel M.A., Fleming H.P.: Selection of lactic acid bacteria for use in vegetable fermentations.Food Microbiol.1, 303–313 (1984).
Daeschel M.A., McKenney M.C., McDonald L.C.: Bacteriocidal activity ofLactobacillus plantarum C-11.Food Microbiol.7, 91–98 (1990).
Farber J.M., Peterkin P.I.:Listeria monocytogenes, a food-borne pathogen.Microbiol. Rev.55, 476–511 (1991).
Fricourt B.V., Barefoot S.F., Testin R.F., Hayasaka S.S.: Detection and activity of plantaricin F, an antibacterial substance fromLactobacillus plantarum BF001 isolated from processed channel catfish.J. Food Protect.57, 678–702 (1994).
Harris L., Daeschel M.A., Stiles M.E., Klaenhammer T.R.: Antimicrobial activity of lactic acid bacteria againstListeria monocytogenes.J. Food Protect.52, 384–387 (1989).
Heinonen T., Kaukanen E., Huttunen T., Tiusanen T., Tuunanen J., Vanne L., Partanen P.: Bioscreen, automated analyser for microbiology, p. 688 in A. Balows, R.C. Tilton A. Turano (Eds).Rapid Method and Automation in Microbiology and Immunology. Brixia Academic Press Brescia 1989.
Hurst A.: Nisin, pp. 85–123 in D. Perlman, A.I. Laskin (Eds):Advances in Applied Microbiology. Academic Press, New York (1981).
Klaenhammer T.R.: Bacteriocins of lactic acid bacteria.Biochimie70. 337–349 (1988).
Konisky J.: Colicins and other bacteriocins with established mode of action.Ann. Rev. Microbiol.36, 125–144 (1982).
Lewus C.B., Montville T.J.: Further characterization of plantaricin BN, bavaricin MN and pediocin A.Food Biotechnol.6, 153–174 (1992).
Mattila T.: Automated turbidimetry—a method for enumeration of bacteria in food samples.J. Food Protect.50, 640–642 (1987).
Montville T.J., Kaiser A.L.: Antimicrobial proteins: Classification, nomenclature, diversity and relationship of bacteriocins, pp. 1–22 inBacteriocins of Lactic Acid Bacteria (D.G. Hoover, L.R. Steenson, Eds). Academic Press, London-New York (1993).
Motlagh A.M., Johnson M.C., Ray B.: Viability loss of food-borne pathogens by starter culture metabolites.J. Food Protect.54, 873–874 884 (1993).
Olasupo N.A., Olukoya D.K., Odunfa S.A.: Plasmid profiles of bacteriocin-producingLactobacillus isolates from African fermented foods.Folia Microbiol.39, 181–186 (1994).
Olasupo N.A., Olukoya D.K., Odunfa S.A.: Studies on bacteriocinogenicLactobacillus isolates from selected Nigerian fermented foods.J. Basic Microbiol.35, 319–324 (1995).
Schillinger U., Lucke F.L.: Identification of lactobacilli from meat and meat products.Food Microbiol.4, 199–208 (1987).
Schillinger U., Lucke F.L.: Antimicrobial activity ofLactobacillus sake isolated from meat.Appl. Environ. Microbiol.55, 1901–1906 (1989).
Sneath P.H.A.,Bergey’s Manual of Systematic Bacteriology, Vol. 2. The Williams & Wilkins Co., Baltimore 1986.
Tagg J.R., Dajani A.S., Wannamaker L.W.: Bacteriocins of Gram-positive bacteria.Bacteriol. Rev.40, 722–756.
Tanasupwat S., Ezaki T., Suzuki K., Okada S., Komagata K., Kozaki M.: Characterization and identification ofLactobacillus pentosus andLactobacillus plantarum strains from fermented foods in Thailand.J. Gen. Appl. Microbiol.38, 121–134 (1992).
Venus J., Idler F., Albrecht C.: New ways of selecting lactic acid bacteria for biotechnological processesAppl. Environ. Microbiol.37, 240–243 (1992).
Vignolo G.M., Suriani F., Ruiz-Holgado A.P., Oliver G.: Antibacterial activity ofLactobacillus strains isolated from dry fermented sausages.J. Appl. Bacteriol.75, 344–349 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olasupo, N.A. Inhibition ofListeria monocytogenes by plantaricin NA, an antibacterial substance fromLactobacillus plantarum . Folia Microbiol 43, 151–155 (1998). https://doi.org/10.1007/BF02816501
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02816501