SpringerLink
Log in

A simple colorimetric assay for muramic acid and lactic acid

  • Original Articles
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The Barker and Summerson method of assaying lactic acid colorimetrically is modified to provide a simple and fast method of measuring lactic acid and other compounds such as muramic acid and glyceraldehyde that will release acetaldehyde on incubation in hot sulfuric acid. The assay can be done with open tubes and no more complicated equipment than a spectrophotometer. A further modification allows a relatively specific determination of formaldehyde.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Figenschou, D. L. and Marais, J. P. (1991),Anal. Biochem. 195, 308–312.

    Article  CAS  Google Scholar 

  2. Hart, S. P. and Doyle, J. J. (1985),J. Anim. Sci. 61, 975–984.

    Google Scholar 

  3. Tusseau, D. and Benoit, C. (1987),J. Chromatog. 395, 323–333.

    Article  CAS  Google Scholar 

  4. Hongo, M., Nomura, Y., and Iwahara, M. (1986),Appl. Environ. Microbiol. 52, 314–319.

    CAS  Google Scholar 

  5. Sato, K. and Tanaka, S. (1990),Anal. Chim. Acta 236, 459–462.

    Article  CAS  Google Scholar 

  6. Asabe, Y., Kojima, S., Suzuki, M., and Takitani, S. (1977),Anal. Biochem. 79, 73–82.

    Article  CAS  Google Scholar 

  7. Teunissen, M. J., Marras, S. A. E., Op den Camp, H. J. M., and Vogels, G. D. (1989),J. Microbiol. Meth. 10, 247–254.

    Article  CAS  Google Scholar 

  8. Barker, S. B. and Summerson, W. H. (1941),J. Biol. Chem. 138, 535–554.

    CAS  Google Scholar 

  9. Moe, A.J. and Carr, S. B. (1985),J. Dairy Sci. 68, 2220–2226.

    Google Scholar 

  10. Singh, R., Kamra, D.N., and Jakhmola, R. C. (1985),Anim. Feed. Sci. Technol. 12, 133–139.

    Article  Google Scholar 

  11. Mimura, T. and Romano, J.-C. (1985),Appl. Environ. Microbiol. 50, 229–237.

    CAS  Google Scholar 

  12. Moriarity, D. J. W. (1977),Oecologia 26, 317–323.

    Article  Google Scholar 

  13. Tipper, D. J. (1968),Biochem. 7, 1441–1449.

    Article  CAS  Google Scholar 

  14. Millar, W. N. and Casida, L. E. (1970),Can. J. Microbiol. 16, 299–304.

    Article  CAS  Google Scholar 

  15. Markus, R. L. (1950),Arch. Biochem. 29, 159–165.

    CAS  Google Scholar 

  16. Hadzija, O. (1974),Anal. Biochem. 60, 512–517.

    Article  CAS  Google Scholar 

  17. King, J. D. and White, D. C. (1977),Appl. Environ. Microbiol. 33, 777–783.

    CAS  Google Scholar 

  18. Walker, S. G., Flemming, C. A., Ferris, F. G., Beveridge, T. J., and Bailey, G. W. (1989),Appl. Environ. Microbiol. 55, 2976–2984.

    CAS  Google Scholar 

  19. Rao, P. and Pattabiraman, T. N. (1989),Anal. Biochem. 181, 18–22.

    Article  CAS  Google Scholar 

  20. Rao, P. and Pattabiraman, T. N. (1990),Anal. Biochem. 189, 178–181.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Animal and Poultry Science, University of Guelph, N1G 2W1, Guelph Ontario, Canada

    Kimberley A. C. C. Taylor

Authors
  1. Kimberley A. C. C. Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taylor, K.A.C.C. A simple colorimetric assay for muramic acid and lactic acid. Appl Biochem Biotechnol 56, 49–58 (1996). https://doi.org/10.1007/BF02787869

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02787869

Index Entries

Search

Navigation