Summary
Tracheary element differentiation in cultured explants of pith parenchyma isolated from heads of romaine lettuce (Lactuca sativa L. var. Romana) was strongly inhibited by concentrations of gentamicin sulfate recommended for tissue culture media (50 to 100 μg/ml). Similar results were obtained with cultured explants of Jerusalem artichoke tuber (Helianthus tuberosus L.). Callus formation was suppressed in the presence of increasing levels of gentamicin sulfate in both tissue systems. Plant tissue culture media employed in studies on cell division and xylem differentiation should be supplemented with this antibiotic in concentrations of 10 μg/ml or less according to these results.
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Dodds, J.H., Roberts, L.W. Some inhibitory effects of gentamicin on plant tissue cultures. In Vitro 17, 467–470 (1981). https://doi.org/10.1007/BF02633507
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DOI: https://doi.org/10.1007/BF02633507