Summary
Ovarian (OM) and extraovarian (EM) mesothelia represent a common source of gynecologic malignancies with yet unclear pathogenesis. Ovulation triggers a finite wave of DNA synthesis and morphogenesis only in native OM cells, probably through the activation of intraovarian growth factors. To evaluate their growth response to such factors, OM and EM cells were obtained from estrous New Zealand white rabbits by enzymatic dispersion and unit gravity sedimentation. Cell cultures were maintained in serumless, fibronectin-rich, HL-1 medium without or with rabbit corpora lutea tissue extracts (CLE). The growth effects of CLE were evaluated by measuring percent changes in cell number relative to controls (CCN), cell population doublings (CPD), cell population doubling time in hours (CPDT). After 7.5 days, CLE enhanced (P<0.001) the growth of both OM and EM cells, which exhibited, respectively, a CCN of 214 and 257%; a CPD of 2.89 and 2.87; and a CPDT of 54.39 and 59.49. CLE-treated OM and EM cells were smaller, formed more cohesive monolayers, and exhibited more frequent and diffuse microvilli than control cells. These data show a similar in vitro response of OM and EM cells to luteal growth factors, suggesting that the lack of postovulatory morphogenesis in native extraovarian mesothelia is due to the spatially restricted activity of intraovarian growth factors.
Similar content being viewed by others
References
Adashi, E. Y.; Rohan, R. M. Intraovarian regulation. Peptidergic signaling systems. TEM 3:243–247; 1992.
Anderson, E.; Lee, G.; Letourneau, R., et al. Cytological observations of the ovarian epithelium of mammals during the reproductive cycle. J. Morphol. 150:135–166; 1976.
Auersperg, N.; Siemens, C. H.; Myrdal, S. E. Human ovarian surface epithelium in primary culture. In Vitro 20:743–775; 1984.
Auersperg, N.; Maclaren, I. A.; Kruk, P. A. Ovarian surface epithelium: autonomous production of connective tissue-type extracellular matrix. Biol. Reprod. 44:717–724; 1991.
Bell, D. A.; Scully, R. E. Benign and borderline serous lesions of the peritoneum in women. Pathol. Annu. 2:1–21; 1989.
Berchuck, A.; Olt, G. J.; Everitt, L., et al. The role of peptide growth factors in epithelial ovarian cancer. Obstet. Gynecol. 75:255–262; 1990.
Bermudez, E.; Everitt, J.; Walter, C. Expression of growth factor and growth factor receptor RNA in pleural mesothelial cells in culture. Exp. Cell Res. 190:91–98; 1990.
Bjersing, L.; Cajander, S. Ovulation and the role of the ovarian surface epithelium. Experimentia 31:605–608; 1975.
Blaustein, A.; Hyun, L. Surface cells of the ovary and pelvic peritoneum: a histochemical and ultrastructural comparison. Gynecol. Oncol. 8:34–43; 1979.
Blaustein, A. Surface (germinal) epithelium and related ovarian neoplasm. Pathol. Annu. 16:247–294; 1981.
Bradford, M. N. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing a principle of protein-dye binding. Ann. Biochem. 72:238–254; 1976.
Gillett, W. R.; Mitchell, A.; Hurst, P. R. A scanning electron microscopic study of the human ovarian surface epithelium: characterization of two cell types. Hum. Reprod. 6:645–650; 1991.
Gondos, B. Surface epithelium of the develo** ovary. Am. J. Pathol. 81:303–320; 1975.
Gorospe, W. C.; Spangelo, B. L. Interleukin-6 production by rat granulosa cells in vitro: effects of cytokines, follicle-stimulating hormone, and cyclic, 3′-5′-adenosine monophosphate. Biol. Reprod. 48:538–543; 1993.
Gospodarowicz, D.; Plouet, J.; Fujii, D. K. Ovarian germinal epithelial cells respond to basic fibroblast growth factor and express its gene: implications for early folliculogenesis. Endocrinology 125:1266–1276; 1989.
Hamilton, T. C. Ovarian cancer, part I. Biol. Curr. Prob. Cancer 41:5–57; 1992.
Hamilton, T. C.; Davies, P.; Griffiths, K. Steroid-hormone receptor status in the normal and neoplastic ovarian surface germinal epithelium. In: Greenwald, G. S.; Terranova, C. F., eds. Factors regulating ovarian function (1983). New York: Raven Press;:81–85.
Kimura, A.; Koga, S.; Kudoh, H., et al. Peritoneal mesothelial cell injury factors in rat cancerous ascites. Cancer Res. 45:4330–4333; 1985.
Motta, P.; Van Blerkom, J.; Makabe, S. Changes in the surface morphology of ovarian germinal epithelium during the reproductive cycle in some pathological conditions. J. Submicrosc. Cytol. 12:407–415; 1980.
Motta, P. M.; Van Blerkom, J. Scanning electron microscopy of the mammalian ovary. In: Motta, P. M.; Hafez, E. S. E., eds. Biology of the ovary. New York: Martinus Nijhoff; 1980:162–175.
Mills, G. B.; May, C.; Hill, M., et al. Ascitic fluid from human ovarian cancer patients contains growth factors necessary for intraperitoneal growth of human ovarian adenocarcinoma cells. J. Clin. Invest. 86:851–855; 1990.
Nicosia, S. V. In vivo and in vitro models for investigating growth and morphogenesis in ovarian mesothelia. Lab. Invest. 64:59a; 1991.
Nicosia, S. V. Morphological changes on the human ovary throughout life. In: Serra, G. B., ed. The ovary. New York: Raven Press; 1983:57–81.
Nicosia, S. V.; Johnson, J. H. Surface morphology of ovarian mesothelium (surface epithelium) and of other pelvic and extrapelvic mesothelial sites in the rabbit. Int. J. Gynecol. Pathol. 3:249–260; 1984.
Nicosia, S. V.; Johnson, J. H.; Streibel, E. J. Isolation and ultrastructure of rabbit ovarian mesothelium (surface epithelium). Int. J. Gynecol. Pathol. 3:348–360; 1984.
Nicosia, S. V.; Johnson, J. H.; Streibel, E. J. Growth characteristics of rabbit ovarian mesothelial (surface epithelial) cells. Int. J. Gynecol. Pathol. 4:58–74; 1985.
Nicosia, S. V.; Narconis, R. J.; Saunders, B. O. Regulation and temporal sequence of surface epithelium morphogenesis in the postovulatory rabbit ovary. Prog. Clin. Biol. Res. 296:111–119; 1989.
Nicosia, S. V.; Nicosia, R. F. Neoplasms of the ovarian mesothelium. In: Azar, H. A., ed. Pathology of human neoplasms. New York: Raven Press; 1988:435–486.
Nicosia, S. V.; Saunders, B. O. Initial characterization of a luteal growth factor for ovarian mesothelial cells. In: Hirshfield, A. H., ed. Growth factors and the ovary. New York: Plenum Press; 1989:237–244.
Nicosia, S. V.; Saunders, B. O.; Acevedo-Duncan, M. E., et al. Biopathology of ovarian mesothelium. In: Familiari, G.; Makabe, S.; Motta, P. M., eds. Ultrastructure of the ovary. New York: Kluwer Academic Publishers; 1991:287–310.
Niebdala, M. J.; Crickard, K.; Bernacki, R. J. Interactions of human ovarian tumor cells with human mesothelial cells grown on extracellular matrix. Anin vitro model system for studying tumor cell adhesion and invasion. Exp. Cell Res. 160:499–513; 1985.
Osterholzer, H. O.; Johnson, H. J.; Nicosia, S. V. An autoradiographic study of rabbit ovarian surface epithelium before and after ovulation. Biol. Reprod. 33:247–258; 1985.
Osterholzer, H. O.; Streibel, E. J.; Nicosia, S. V. Effect of protein hormones on ovarian surface epithelial cells. Biol. Reprod. 33:247–258; 1985.
Piquette, G. N.; Timms, B. G. Isolation and characterization of rabbit ovarian surface epithelium, granulosa cells, and peritoneal mesothelium in primary culture. In Vitro Cell. Dev. Biol. 26:471–481; 1990.
Porter, K.; Prescott, D.; Frye, J. Changes in surface morphology of Chinese hamster ovary cells during the cell cycle. J. Cell Biol. 57:815–836; 1973.
Setrakian, S. H.; Saunders, B. O.; Nicosia, S. V. Isolation and characterization of rabbit peritoneal mesothelial cells. Acta Cytol. 34:(1)92–100; 1990.
Van Blerkom, J.; Motta, P. The cellular basis of mammalian reproduction. Baltimore: Urban and Schwarzenberg; 1979.
Vergara, J.; Ingram, P.; Stone, K. Microvilli and cell associationin vitro. Scanning Electron Microsc. 2:111–117; 1977.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Setrakian, S., Oliveros-Saunders, B. & Nicosia, S.V. Growth stimulation of ovarian and extraovarian mesothelial cells by corpus luteum extract. In Vitro Cell Dev Biol - Animal 29, 879–883 (1993). https://doi.org/10.1007/BF02631367
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02631367