Abstract
The zinc content of trabecular and cortical bone was measured in 28 femora obtained at autopsy. The ratios of calcium to zinc, phosphorus and magnesium were determined and their relation to age and vertebral density was investigated. The calcium/zinc ratio was significantly higher in cortical as opposed to trabecular bone, whereas the calcium/phosphorus ratios were similar. There was a significant decrease in the calcium/zinc ratios of both trabecular and cortical bone with age and the former was directly related to vertebral density. The calcium/phosphorus ratios of trabecular and cortical bone did not vary significantly with age or vertebral density. Magnesium/zinc ratios tended to parallel the calcium/zinc ratios. The calcium/magnesium ratios were significantly higher in cortical as opposed to trabecular bone. None of these ratios appeared to be affected by the sex of the subject. These findings suggest that changes in skeletal zinc content with age are related to alterations in bone morphology, and that osteoporosis in man develops in association with zinc accumulation rather than zinc deficiency.
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Aitken, J. M.: Mineral metabolism and the ovary. M. D. Thesis Cambridge University (1973)
Aitken, J. M., Lindsay, R., Hart D. M.: Plasma zinc in pre-and post-menopausal women: its relationship to oestrogen therapy. Clin. Sci.44, 91–94 (1973)
Alfrey, A. C., Miller, N. L.: Bone magnesium pools in uremia. J. clin. Invest.52, 3019–3027 (1973)
Asling, C.W., Hurley, L. S.: The influence of trace elements on the skeleton. Clin. Orthop.27, 213–262 (1963)
Barnett, E., Nordin, B. E. C.: The radiological diagnosis of osteoporosis: a new approach. Clin. Radiol.11, 166–174 (1960)
Bauer, W., Aub, J. C., Albright, F.: Studies of calcium and phosphorus metabolism. J. exp. Med.49, 145–161 (1929)
Briggs, A. P.: A modification of the Bell-Doisy phosphate method. J. biol. Chem.53, 13–16 (1922)
Briggs, M. H., Briggs, M., Austin, J.: Effects of steroid pharmaceuticals on plasma zinc. Nature (Lond.)232, 480–481 (1971)
Contiguglia, S. R., Alfrey, A. C., Miller, W., Butkus, D.: Total body magnesium excess in chronic renal failure. Lancet1970 I, 1300–1302
Ferguson, H. W., Leaver, A. G.: The effects of diets high in zinc at different levels of calcium and vitamin D on the rat humerus and incisor. Calcif. Tiss. Res.8, 265–275 (1972)
Fernandez-Madrid, F., Prasad, A. S., Oberleas, D.: Effect of zinc deficiency on collagen metabolism. J. Lab. clin. Med.78, 853 (1971)
Haumont, S.: Distribution of zinc in bone tissue. J. Histochem. Cytochem.9, 141–145 (1961)
Lutz, R. E.: The normal occurrence of zinc in biologic materials; review of literature and study of normal distribution of zinc in rat, cat and man. J. industr. Hyg.8, 177–207 (1926)
Mathies, J. C., Lund, P. K.: X-ray spectrographic microanalysis of human tissues for iron and zinc. Amer. J. clin. Path.59, 417–422 (1973)
McBean, L. D., Dove, J. T., Halsted, J. A., Smith, J. C.: Zinc concentration in human tissues. Amer. J. clin. Nutr.25, 672–676 (1972)
Meema, H. E.: Menopausal and aging changes in muscle mass and bone mineral content. J. Bone Jt Surg.48A, 1138–1144 (1966)
Mitchell, H. H., Hamilton, T. S., Steggerda, F. R., Bean, H. W.: The chemical composition of the adult human body and its bearing on the biochemistry of growth. J. biol. Chem.158, 625–637 (1945)
Morrison, A. B., Sarett, H. P.: Studies on zinc deficiency in the chick. J. Nutr.58, 267–280 (1958)
Neuman, W. F., Mulryan, B. J.: Synthetic hydroxyapatite crystals. IV Magnesium incorporation. Calcif. Tiss. Res.7, 133–138 (1971)
Nordin, B. E. C., Smith, D. A.: Diagnostic procedures in disorders of calcium metabolism. 1st ed. London: Churchill, 1965
O’Dell, B. L., Newberne, P. M., Savage, J. E.: Significance of dietary zinc for the growing chicken. J. Nutr.65, 503–518 (1958)
Prasad, A. S., Oberleas, D.: Zinc in human serum: Evidence for an amino acid bound fraction. J. Lab. clin. Med.72, 1006 (1968)
Prasad, A. S., Oberleas, D., Rajasekaran, G.: Essential micronutrient elements. Biochemistry and changes in liver disorders. Amer. J. clin. Nutr.23, 581–591 (1970)
Sadasivan, V.: Studies on the biochemistry of zinc. I. Effect of feeding zinc on the liver and bones of rats. Biochem. J.48, 527–530 (1951)
Samachson, J., Dennis, J., Fowler, R., Schmitz, A.: The reaction of65Zn with the surfaces of bone and bone mineral. Biochim. biophys. Acta. (Amst.)148, 767–773 (1967)
Sandstead, H. H., Prasad, A. S., Schulert, A. R., Farid, Z., Miale, A., Bassilly, S., Darby, W. J.: Human zinc deficiency, endocrine manifestations and response to treatment. Amer. J. clin. Nutr.20, 422–442 (1967)
Smith, D. A., Anderson, J. B., Shimmins, J., Speirs, C. F., Barnett, E.: Changes in metacarpal mineral content and density in normal male and female subjects with age. Clin. Radiol.20, 23–31 (1969)
Thiers, R. E., Vallee, B. L.: Distribution of metals in subcellular fractions of rat liver. J. biol. Chem.226, 911–920 (1957)
Thompson R. H. S., King, E. J.: Biochemical disorders in human disease. 2nd ed. London: Churchill, 1964
Trudeau, D. L., Freier, E. F.: Determination of calcium in urine and serum by atomic absorption spectrophotometry (AAS). Clin. Chem.13, 101–114 (1967)
Vikbladh, I.: Studies on zinc in blood II. Scand. J. clin. Lab. Invest.3, Suppl. 2 (1951)
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Aitken, J.M. Factors affecting the distribution of zinc in the human skeleton. Calc. Tis Res. 20, 23–30 (1976). https://doi.org/10.1007/BF02546394
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DOI: https://doi.org/10.1007/BF02546394