Abstract
Doubling the length of a CP-Sil 88 capillary column (Chrompack, Middelburg, The Netherlands) from 50 to 100 m remarkably improves the resolution of individualtrans-18:1 isomers from either ruminant fats or partially hydrogenated oils. Although the use of a 50-m column gives interesting results, it does not allow sufficient resolution of thetrans-10 andtrans-11 18:1 isomers. Moreover, thetrans-6 totrans-9 18:1 isomers emerge as a single group of peaks, whereas thetrans-12 isomer is only partly resolved from the adjacenttrans-11 andtrans-13 plustrans-14 isomers. With the 100-m column, thetrans-9,trans-10, andtrans-12 18:1 isomers are almost base-line resolved from other isomers. However, with both columns, it is not possible to separate the critical pair oftrans-13 andtrans-14 18:1 acids which co-elute under a single peak. Despite this minor drawback, the 100-m CP-Sil 88 column appears to be of great interest for the separation and the quantitation of most individualtrans-18:1 acids. Except for the use of argentation thin-layer chromatography, there is no need for complementary techniques, such as ozonolysis. This simple and powerful tool may be applied to ruminant fats, partially hydrogenated oils, and human tissue lipids.
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Wolff, R.L., Bayard, C.C. Improvement in the resolution of individualtrans-18:1 isomers by capillary gas-liquid chromatography: Use of a 100-m CP-Sil 88 column. J Am Oil Chem Soc 72, 1197–1201 (1995). https://doi.org/10.1007/BF02540988
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DOI: https://doi.org/10.1007/BF02540988