SpringerLink
Log in

Determination of ephedra alkaloids by high-performance liquid chromatography

  • Short Communications
  • Column Liquid Chromatography
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Two simple methods were developed for the simultaneous determination of six alkaloids (ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, methylephedrine and methyl-pseudoephedrine) inEphedrae Herba by high-performance liquid chromatography. The first method was carried out by using a Cosmosil 5C18-MS column with a gradient solvent system consisting of a phosphate buffer and acetonitrile, and detection at 210 nm. The contents of alkaloids in non-pretreated ephedra herb extracts could be determined easily in 50 min. Alternatively, the alkaloids could be determined within 35 minutes by using a Cosmosil 5C18-MS column with an isocratic solvent system of a sodium dodecyl sulfate-acetonitrile solution. The two methods are compared and discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Hsu, H.Y.; Chen, Y.P.; Sheu, S.J.; Hsu, C.H.; Chen, C.C.; Cheng, H.C.Chinese Materia Medica-A Concise Guide, Modern Drug Weekly Press, Taipei,1984.

    Google Scholar 

  2. Nagai, W.N.; Kanao, S.J. Pharm. Soc. 1928,48, 845–851.

    CAS  Google Scholar 

  3. Yamazaki, K.Wakan Iyaku Gakkaishi 1985,2, 93–94.

    CAS  Google Scholar 

  4. Kimura, K.; Shimada, H.; Normura, S.Y.; Tanaka, T.Yakugaku Zasshi 1973,93, 364–368.

    CAS  Google Scholar 

  5. Hashimoto, Y.; Ikeshiro, Y.; Higashiyama, T.; Audo, K.; Endo, M.Yakugaku Zasshi 1977,97, 594–596.

    CAS  Google Scholar 

  6. Yamasaki, K.; Fujita, K.; Sakamiti, M.; Okada, K.; Yoshida, M.; Tanaka, O.Chem. Pharm. Bull. 1974,22, 2898–2902.

    CAS  Google Scholar 

  7. Yamasaki, K.; Fujita, K.Chem. Pharm. Bull. 1979,27, 43–47.

    CAS  Google Scholar 

  8. Iwanami, N.; Ohtsuka, Y.; Kubo, H.Yaoxue Tongbao. 1985,20, 149–153.

    CAS  Google Scholar 

  9. Anetai, M.; Yamagishi, T.Hokkaidoritsu Eisei Kenkyushoho 1987,37, 44–47.

    Google Scholar 

  10. Noguchi, M.; Hosoda, K.; Suzuki, H.Yakugaku Zasshi 1987,107, 372–376.

    CAS  Google Scholar 

  11. Imaz, C.; Carrearas, D.; Navajas, R.; Rodriguez, C.; Rodriguez, A.F.; Maynar, J.; Cortes, R.J. Chromatogr. 1993,631, 201–205.

    Article  CAS  Google Scholar 

  12. Sagara, K.; Suto, K.; Ito, Y.; Nagai, Y.; Suzuki, H.; Satake, M.Iyakuhin Kenkyu 1996,27, 255–261.

    CAS  Google Scholar 

  13. Kasahara, Y.; Hikino, H.; Hine, T.J. Chromatogr. 1985,324, 503–507.

    Article  CAS  Google Scholar 

  14. Liu, Y.M.; Sheu, S.J.J. Chromatogr. 1992,600, 370–372.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, National Taiwan Normal University, 107, Taipei, Taiwan

    S. J. Sheu

  2. Department of Pharmacy, Chia Nan University of Pharmacy and Science, 717, Tainan, Taiwan

    M. H. Huang

Authors
  1. S. J. Sheu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. H. Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sheu, S.J., Huang, M.H. Determination of ephedra alkaloids by high-performance liquid chromatography. Chromatographia 54, 117–119 (2001). https://doi.org/10.1007/BF02491844

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02491844

Key Words

Search

Navigation