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Method development and validation for the simultaneous determination of five selective serotonin reuptake inhibitors by capillary zone electrophoresis

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Summary

A capillary zone electrophoresis method is proposed for the separation of five antidepressants. Optimum conditions for the separation were investigated. A background electrolyte solution consisting of 40 mM phosphate buffer adjusted to pH 2.5, hydrodynamic injection, and a 28 kV separation voltage were used. Relative standard deviations (RSD) were <0.9% and <1.7% for migration time and corrected peak area (n=21), respectively. The detection limits for the five antidepressants ranged from 0.3 to 0.7 mg L−1. The stability of the solutions, linearity, accuracy, and precision were examined during validation of the method. The method is rapid and sensitive, when it was tested for the analysis of pharmaceutical formulations the recoveries obtained were between 98 and 103% of the nominal content.

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References

  1. Viala, A.Actualités Psychiatriques 1982,4, 65.

    Google Scholar 

  2. Kasper, S.; Höflich, G.; Scholl, H.P.; Möller, H.J.Hum. Psychopharmacol 1994,9, 1–8.

    Article  CAS  Google Scholar 

  3. Preskorn, S.H.J. Clin. Psychiatry 1994,55 (Suppl A) 6–12.

    Google Scholar 

  4. Berzas, J.J.; Villaseñor, M.J.; Contento, A.M., Aguas, E.J. Chromatogr. Sci. 2000,38, 200–206.

    Google Scholar 

  5. Alvarez, J.C.; Bothue, D.; Collignon, I.; Advenier, C.; Spreus-Varoquaux, O.J. Chromatogr. B 1998,707, 175–180.

    CAS  Google Scholar 

  6. Lantz, R.J.; Farid, K.Z.; Koons, J.; Tenbargue, J.B.; Bopp, R.J.J. Chromatogr. 1993,614, 175–179.

    CAS  Google Scholar 

  7. Bagli, M.; Rao, M.L.; Sobanski, T.; Laux, G.J. Liq. Chromatogr. Rel. Technol. 1997,20, 283–295.

    CAS  Google Scholar 

  8. Eap, C.B.; Baumann, P.J. Chromatogr. B 1996,686, 51–63.

    CAS  Google Scholar 

  9. Berzas, J.J.; Contento, A.M.; Villaseñor, M.J.; Aguas, E.Anal. Chim. Acta 2000,417, 169–176.

    Article  Google Scholar 

  10. Desiderio, C.; Rudaz, S.; Raggli, M.A.; Fanali, S.Electrophoresis 1999,20(17), 3432–3438.

    Article  CAS  Google Scholar 

  11. Halvorsen, T.G.; Pedersen-Bjergaard, S.; Rasmussen, K.E.J. Chromatogr. A 2001,909(1), 87–93.

    Article  CAS  Google Scholar 

  12. European Pharmacopeia, 3rd Edn,1997.

  13. Kuhn, R.; Hoffstetter-Kuhn, S.Capillary Electrophoresis: Principles and Practice, Springer, Paris,1993.

    Google Scholar 

  14. Huber, L.Applications of Diode-Array Experience, Hewlett-Packard publication no. 12-5953-2330,1989.

Download references

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Authors and Affiliations

  1. Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, 13071, Ciudad Real, Spain

    J. J. Berzas Nevado & A. M. Contento Salcedo

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  1. J. J. Berzas Nevado
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  2. A. M. Contento Salcedo
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Nevado, J.J.B., Salcedo, A.M.C. Method development and validation for the simultaneous determination of five selective serotonin reuptake inhibitors by capillary zone electrophoresis. Chromatographia 55, 369–373 (2002). https://doi.org/10.1007/BF02491674

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  • DOI: https://doi.org/10.1007/BF02491674

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