Summary
Momordicoside A has been determined by solid-phase extraction (SPE) on a Envi Carb cartridge (3 mL, 250mg) then high-performance liquid chromatography (HPLC) on a C18 column (4.6 mm×250 mm, 5 μm particle) with acetonitrile-methanol-50mm potassium dihydrogen phosphate buffer, 25:20:60 (v/v), as mobile phase, at a flow rate of 0.8 mL min−1, and UV detection at 208 nm. The analytical method was shown to be highly reproducible, with precision (asRSD) and accuracy (asRME)<10%, both intra-day and inter-day. Absolute recoveries were >90%. The method was applied to the determination of momordicoside A in various tissues from different varieties of bitter melon from different producing areas.
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Wang, S., Tang, L., Guo, Y. et al. Determination of momordicoside A in bitter melon by high-performance liquid chromatography after solid-phase extraction. Chromatographia 53, 372–374 (2001). https://doi.org/10.1007/BF02491069
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DOI: https://doi.org/10.1007/BF02491069