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Combined immunophenoty** and FISH with sex chromosome-specific DNA probes for the detection of chimerism in epidermal Langerhans cells after sex-mismatched bone marrow transplantation

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Abstract

Langerhans cells (LC) of the skin represent bone marrow-derived dendritic antigen-presenting cells and are therefore important in pathophysiological processes such as rejection, graft-versus-host disease, and graft-versus-leukemia-reaction after bone marrow transplantation (BMT). For understanding of these diseases, the evaluation of the chimeric status of LC following BMT is of great interest. To analyze the sex chromosome constitution of LC in the skin, we established a modified and refined technique of combined immunophenoty** and fluorescence in situ hybridization (FISH) and investigated frozen sections of skin biopsies from nine patients after allogeneic sex-mismatched BMT and of two healthy donors for control. LC were specifically labeled using a fluorescent CD1 a antibody and hybridized simultaneously with X and Y chromosome-specific DNA probes. The results of this practical application on nine leukemia patients show the appearance of donor-type LC and the persistence of host-type LC at various times (36 up to 1395 days) after sex-mismatched BMT. Complete chimerism of LC could not be detected in any case. The frequency of recipient-specific LC ranged from 7% to 92% and showed no correlation with time postgafting. We conclude from our results of 1461 analyzed LC that combined immunophenoty** and interphase cytogenetic analysis by FISH is the method of choice for the assessment of chimerism in a particular cell type after sex-mismatched BMT. Its practical application on other tissues affected by BMT-related pathophysiological processes reveals further knowledge of the time-dependent course of chimeric patterns after BMT.

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Authors and Affiliations

  1. GSF-National Research Center for Environment and Health, Institute of Radiobiology, Postfach 1129, D-85758, Oberschleißheim, Germany

    Harald Hessel, Horst Zitzelsberger & Manfred Bauchinger

  2. GSF-National Research Center for Environment and Health, Institute of Clinical Haematology, Germany

    Johann Mittermüller

  3. Strahlenbiologisches Institut, Ludwig Maximilians Universität München, Germany

    Horst Zitzelsberger

  4. Life Science Division, Lawrence Berkeley Laboratory, Center for Molecular Cytogenetics, Berkeley, California, USA

    Heinz-Ulrich Weier

Authors
  1. Harald Hessel
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  2. Johann Mittermüller
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  3. Horst Zitzelsberger
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  4. Heinz-Ulrich Weier
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  5. Manfred Bauchinger
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Hessel, H., Mittermüller, J., Zitzelsberger, H. et al. Combined immunophenoty** and FISH with sex chromosome-specific DNA probes for the detection of chimerism in epidermal Langerhans cells after sex-mismatched bone marrow transplantation. Histochem Cell Biol 106, 481–485 (1996). https://doi.org/10.1007/BF02473310

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