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Chloride secretion by canine tracheal epithelium: IV. Basolateral membrane K permeability parallels secretion rate

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We evaluated the K conductance properties of the basolateral membranes of the surface cells of canine tracheal epithelium using microelectrode techniques. Studies were conducted under basal conditions (indomethacin, 10−6 m, mucosal solution) and after stimulation of electrogenic Cl secretion with epinephrine (10−6 m, serosal solution). Elevated serosal solution [K] depolarized the electrical potential differences across the apical (ψa) and basolateral (ψb) membranes in both the presence and absence of epinephrine. Serosal barium (0.5mm) also depolarized ψa and ψb and selectively increased basolateral membrane resistance threefold. We also used K-selective microelectrodes to determine cell K activity (a K c ) and the driving force for K transport across the limiting membranes under basal and stimulated conditions. Stimulation of Cl secretion was not associated with significant changes in ψb ora K c so that the driving force for k exit from cell to serosal solution (ca. 20 mV) was not altered. There was close agreement between the basolateral membrane electromotive force (E b ) determined from prior studies (M.J. Welsh, P.L. Smith and R.A. Frizzell,J. Membrane Biol. 71:209–218, 1983) and the chemical potential difference for K across this barrier (E K b ) in the presence and absence of epinephrine. These findings support the notion that the basolateral membrane is characterized by a high conductance to K under both secreting and nonsecreting conditions and indicate that the decrease in basolateral membrane resistance that accompanies stimulation of Cl secretion results from an increase in its K conductance. This obviates changes ina K c , that would otherwise accompany increased Na/K pump activity and, by hyperpolarizing ψa establishes the electrical driving force for Cl secretion across the apical membrane.

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Authors and Affiliations

  1. Department of Physiology, University of Kansas Medical Center, 66103, Kansas City, Kansas

    Philip L. Smith & Raymond A. Frizzell

  2. Department of Physiology and Biophysics, University of Alabama in Birmingham, 35294, Birmingham, Alabama

    Philip L. Smith & Raymond A. Frizzell

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  1. Philip L. Smith
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  2. Raymond A. Frizzell
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Smith, P.L., Frizzell, R.A. Chloride secretion by canine tracheal epithelium: IV. Basolateral membrane K permeability parallels secretion rate. J. Membrain Biol. 77, 187–199 (1984). https://doi.org/10.1007/BF01870568

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  • DOI: https://doi.org/10.1007/BF01870568

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