SpringerLink
Log in

Epitope map** of capsid proteins VP2 and VP3 of infectious bursal disease virus

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Twenty hybridoma cell lines producing monoclonal antibodies (MAbs) against serotype 1 infectious bursal disease virus (IBDV) of GBF-1 and the attenuated GBF-1E strains were produced. The MAbs recognized major structural proteins VP2 and VP3. MAb recognition sites were mapped using recombinantEscherichia coli clones which expressed N-terminal and (or) C-terminal truncated virus antigens, and competitive-binding assays. At least 3 conformation-dependent serotype 1 specific virus neutralizing antigenic sites and a linear antigenic site were defined on VP2 and VP3, respectively. Two of the conformational virus neutralizing antigenic sites were localized in the central area of VP2 consisting of 156 amino acid residues, and the linear epitope was localized in C-terminal 105 amino acid residues of VP3. Another conformational virus neutralizing antigenic site recognized with the virus neutralizing MAb GK-5 was not defined because GK-5 did not react with virus antigen expressed in recombinantE. coli. The conformational antigenic site was supposed to be composed of tertiary or quaternary protein structure, which may not be constructed in recombinantE. coli.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Canada)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Azad AA, Barrett SA, Fahey KJ (1985) The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology 143: 35–44

    Google Scholar 

  2. Azad AA, Jagadish MN, Brown MA, Hudson PJ (1987) Deletion map** and expression inEscherichia coli of the large genomic segment of a birnavirus. Virology 161: 145–152

    Google Scholar 

  3. Bayliss CD, Spies U, Shaw K, Peters RW, Papageorgiou A, Müller H, Boursnell ME (1990) A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. J Gen Virol 71: 1303–1312

    Google Scholar 

  4. Becht H, Müller H, Müller HK (1988) Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. J Gen Virol 69: 631–640

    Google Scholar 

  5. Chettle NJ, Eddy RK, Wyeth PJ (1985) The isolation of infectious bursal disease virus from turkeys in England. Br Vet J 141: 141–145

    Google Scholar 

  6. Dobos P, Hill BJ, Hallett R, Kells DT, Becht H, Teninges D (1979) Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. J Virol 32: 593–605

    Google Scholar 

  7. Fahey KJ, McWaters P, Brown MA, Erny K, Murphy VJ, Hewish DR (1991) Virusneutralizing and passively protective monoclonal antibodies to infectious bursal disease virus of chickens. Avian Dis 35: 365–373

    Google Scholar 

  8. Fahey KJ, O'Donnell IJ, Azad AA (1985) Characterization by Western blotting of the immunogens of infectious bursal disease virus. J Gen Virol 66: 1479–1488

    Google Scholar 

  9. Heine HG, Haritou M, Failla P, Fahey K, Azad A (1991) Sequence analysis and expression of the host-protective immunogen VP2 of a variant strain of infectious bursal disease virus which can circumvent vaccination with standard type I strains. J Gen Virol 72: 1835–1843

    Google Scholar 

  10. Hirai K, Shimakura S (1974) Structure of infectious bursal disease virus. J Virol 14: 957–964

    Google Scholar 

  11. Hirai K, Shimakura S, Chang C, Adachi Y, Kawamoto E (1973) Isolation of infectious bursal disease virus and distribution of precipitating antibodies in chicken sera. Jpn J Vet Sci 35: 105–113

    Google Scholar 

  12. Hirai K, Shimakura S, Kawamoto E, Taguchi F, Kim ST, Chang CN, Iritani Y (1974) The immunodepressive effect of infectious bursal disease virus in chickens. Avian Dis 18: 50–57

    Google Scholar 

  13. Hudson PJ, McKern NM, Power BE, Azad AA (1986) Genomic structure of the large RNA segment of infectious bursal disease virus. Nucleic Acids Res 14: 5001–5012

    Google Scholar 

  14. Ismail NM, Saif YM, Moorhead PD (1988) Lack of pathogenicity of five serotype 2 infectious bursal disease viruses in chickens. Avian Dis 32: 757–759

    Google Scholar 

  15. Izawa H, Eiguchi Y, Nagabayashi T (1978) Attenuation of infectious bursal disease virus by serial passage through chicken embryonated eggs and chicken and duck embryonic fibroblasts (Japanese). Uirusu 28: 41–45

    Google Scholar 

  16. Jackwood DH, Saif YM (1987) Antigenic diversity of infectious bursal disease viruses. Avian Dis 31: 766–770

    Google Scholar 

  17. Jackwood DJ, Saif YM, Hughes JH (1982) Characteristics and serologic studies of two serotypes of infectious bursal disease virus in turkeys. Avian Dis 26: 871–882

    Google Scholar 

  18. Jagadish MN, Azad AA (1991) Localization of a VP3 epitope of infectious bursal disease virus. Virology 184: 805–807

    Google Scholar 

  19. Jagadish MN, Staton VJ, Hudson PJ, Azad AA (1988) Birnavirus precursor polyprotein is processed inEscherichia coli by its own virus-encoded polypeptide. J Virol 62: 1084–1087

    Google Scholar 

  20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    Google Scholar 

  21. Lee LH (1990) Monoclonal antibodies against different epitopes of a 40 Kd capsid protein of infectious bursal disease viruses. Proc Natl Sci Counc B ROC 14: 75–84

    Google Scholar 

  22. Mahardika GNK, Becht H (1995) Map** of cross-reacting and serotype-specific epitopes on the VP3 structural protein of the infectious bursal disease virus (IBDV). Arch Virol 140: 765–774

    Google Scholar 

  23. McFerran JB, McNulty MS, McKillop ER, Connor TJ, McCracken RM, Collins DS, Allan GM (1980) Isolation and serological studies with infectious bursal disease viruses from fowl, turkeys and ducks: demonstration of a second serotype. Avian Pathol 9: 395–404

    Google Scholar 

  24. Müller H, Scholtissek C, Becht H (1979) The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. J Virol 31: 584–589

    Google Scholar 

  25. Mundt E, Beyer J, Müller H (1995) Identification of a novel viral protein in infectious bursal disease virus-infected cells. J Gen Virol 76: 437–443

    Google Scholar 

  26. Nick H, Cursiefen D, Becht H (1976) Structural and growth characteristics of infectious bursal disease virus. J Virol 18: 227–234

    Google Scholar 

  27. Öppling V, Müller H, Becht H (1991) Heterogeneity of the antigenic site responsible for the induction of neutralizing antibodies in infectious bursal disease virus. Arch Virol 119: 211–223

    Google Scholar 

  28. Öppling V, Müller H, Becht H (1991) The structural polypeptide VP3 of infectious bursal disease virus carries group- and serotype-specific epitopes. J Gen Virol 72: 2275–2278

    Google Scholar 

  29. Reddy SK, Silim A (1991) Comparison of neutralizing antigens of recent isolates of infections bursal disease virus. Arch Virol 117: 287–296

    Google Scholar 

  30. Saif YM (1991) Immunosuppression induced by infectious bursal disease virus. Vet Immunol Immunopathol 30: 45–50

    Google Scholar 

  31. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  32. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  33. Schnitzler D, Bernstein F, Müller H, Becht H (1993) The genetic basis for the antigenicity of the VP2 protein of the infectious bursal disease virus. J Gen Virol 74: 1563–1571

    Google Scholar 

  34. Shaw RD, Vo PT, Offit PA, Coulson BS, Greenberg HB (1986) Antigenic map** of the surface proteins of rhesus rotavirus. Virology 155: 434–451

    Google Scholar 

  35. Skeeles JK, Lukert PD, De Buysscher EV, Fletcher OJ, Brown J (1979) Infectious bursal disease viral infections. I. Complement and virus-neutralizing antibody response following infection of susceptible chickens. Avian Dis 23: 95–106

    Google Scholar 

  36. Snyder DB, Lana DP, Cho BR, Marquardt WW (1988) Group and strain-specific neutralization sites of infectious bursal disease virus defined with monoclonal antibodies. Avian Dis 32: 527–534

    Google Scholar 

  37. Yamaguchi S, Imada T, Kawamura H (1981) Growth and infectivity titration of virulent infectious bursal disease virus in established cell lines from lymphoid leukosis tumors. Avian Dis 25: 927–935

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Gifu, Japan

    T. Yamaguchi, K. Iwata, M. Kobayashi, M. Ogawa, H. Fukushi & K. Hirai

Authors
  1. T. Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Iwata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Ogawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Fukushi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. K. Hirai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamaguchi, T., Iwata, K., Kobayashi, M. et al. Epitope map** of capsid proteins VP2 and VP3 of infectious bursal disease virus. Archives of Virology 141, 1493–1507 (1996). https://doi.org/10.1007/BF01718250

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01718250

Keywords

Search

Navigation