Abstract
Transforming growth factors (TGF) are defined as biologically active polypeptides which reversibly confer the transformed phenotype onto untransformed cultured cells. They have been subdivided into two classes: type α and type β TGFs. TGF-β acts synergistically with TGF-α in inducing phenotypic transformation. TGF-β can also act as negative autocrine growth factor. A human 1050-bp EcoRI cDNA fragment was used to map the human locus for TGF-β by Southern blotting of DNA prepared from 17 human × Chinese hamster somatic cell hybrids. The humanspecific restriction fragments segregated with human chromosome 19 in all of 14 informative hybrids. All other human chromosomes were discordant with the TGF-β bands in at least four hybrids. After in situ hybridization of the tritiated TGF-β probe to normal human metaphase spreads, 151 silver grains were scored in 54 cells. Of 24 grains over chromosome 19, 16 grains (11%) lay over region 19q13.1 → q13.3. Of the 54 cells analyzed, 16 (30%) had label over region 19q13.1 → q13.3. Thus,TGFB is assigned to chromosome 19, subbands q13.1 → q13.3. TheTgf- β locus in the mouse was mapped to chromosome 7 by hybridizing a murine cDNA probe to a Chinese hamster × mouse hybrid panel. Human chromosome 19 and proximal mouse chromosome 7 share another four homologous loci.
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Literature cited
Roberts, A.B., Lamb, L.C., Newton, D.L., Sporn, M.B., De Larco, J.E., and Todaro, G.J. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:3494–3498.
Todaro, G.J., Fryling, D., and De Larco, J.E. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:5258–5262.
Marquardt, H., and Todaro, G.J. (1982).J. Biol. Chem. 257:5220–5225.
Marquardt, H., Hunkapiller, M.W., Hood, L.E., and Todaro, G.J. (1984).Science 223:1079–1082.
Derynck, R., Roberts, A.B., Winkler, M.E., Chen, E.Y., and Goeddel, D.V. (1984).Cell 38:287–297.
Massague, J. (1983).J. Biol. Chem. 258:13614–13620.
Assoian, R.K., Komoriya, A., Meyers, C.A., Miller, D.M., and Sporn M.B. (1983).J. Biol. Chem. 258:7155–7160.
Derynck, R., Jarrett, J.A., Chen, E.Y., Eaton, D.H., Bell, J.R., Assoian, E.K., Roberts, A.B., Sporn, M.B., and Goeddel, D.V. (1985).Nature 316:701–705.
Frolik, C.A., Wakefield, L.M., Smith, D.M., and Sporn, M.B. (1984).J. Biol. Chem. 259:10995–11000.
Tucker, R.F., Branum, E.L., Shipley, G.D., Ryan, R.J., and Moses, H.L. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:6757–6761.
Massague, J., and Like, B. (1985).J. Biol. Chem. 260:2636–2645.
Brissenden, J.E., Derynck, R., and Francke, U. (1985).Cancer Res. 45:5593–5597.
Frolik, C.A., Dart, L.L., Meyers, C.A., Smith, D.M., and Sporn, M.B. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:3676–3680.
Roberts, A.E., Anzano, M.A., Meyers, C.A., Wideman, J., Blacher, R., Pan, Y.E., Stein, S., Lehrman, S.R., Smith, J.M., Lamb, L.C., and Sporn, M.B. (1983).Biochemistry 22:5692–5698.
Anzano, M.A., Roberts, A.D., Smith, J.M., Sporn, M.B., and De Larco, J.E. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:6264–6268.
Assoian, R.K., Frolik, C.A., Roberts, A.B., Miller, D.M., and Sporn, M.B. (1984).Cell 36:35–41.
Tucker, R.F., Bolkenant, M.E., Branum, E.L., and Moses, H.L. (1983).Cancer Res. 43:1581–1586.
Tucker, R.F., Shipley, G.D., Moses, H.L., and Holley, R.W. (1984).Science 226:705–707.
Roberts, A.B., Anzano, M.A., Wakefield, L.M., Roche, N.S., Stern, D.F., and Sporn, M.B. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:119–123.
Derynck, R., Jarrett, J., Chen, E.Y., and Goeddel, D.V., (1986).J. Biol. Chem. 261:4377–4379.
Reinberg, A.R., and Vogelstein, B. (1983).Anal. Biochem. 132:6–13.
Francke, U., Busby, N., Shaw, D., Hansen, S., and Brown, M.G. (1976).Somat. Cell Genet. 2:27–40.
Francke, U., and Pellegrino, M.A. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:1147–1151.
Francke, U., and Francke, B. (1981).Somat. Cell Genet. 7:171–191.
Francke, U. (1984).Cytogenet. Cell Genet. 38:298–307.
Southern, E. (1975).J. Mol. Biol. 98:503–507.
Meera Khan P. (1971).Arch. Biochem. Biophys. 145:470–483.
van Someren, H., van Henegouwen, H.B., Los, E., Wurzer-Figurelli, E., Doppert, B., Vervloet, M., and Meera Khan P. (1974).Humangenetik 25:189–191.
Kirsch, I.R., Morton, C.C., Nakahara, K., and Leder, P. (1982).Science 216:301–303.
Harper, M.E., and Saunders, G.F. (1981).Chromosoma 83:431–439.
Francke, U., and Taggart, R.T. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:5230–5233.
Francke, U., Lalley, P.A., Moss, W., Ivy, L., and Minna, J.D. (1977).Cytogenet. Cell Genet. 19:57–84.
Naylor, S., Lalouel J.-M., and Shaw D.J. (1985).Cytogenet. Cell Genet. 40:242–267.
Ueshima, Y., Bird, M.L., Vardiman, J.W., and Rowley, J.D. (1985).Int. J. Cancer 36:287–290.
Bloomfield, C.D., Arthur, D.C., Frizzera, G. Levine, E.G., Peterson, B.A., and Gajl-Peczalska, K.J. (1983).Cancer Res. 43:2975–2984.
Croce, C.M., Shander, M., Martinis, J., Cicurel, L., D'Anconna, G.G., Dolby, T.W., and Koprowski, H. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:3416–3419.
Erikson, J., Finan, J., Nowell, P.C., and Croce, C.M. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:5611–5615.
Dalla-Favera, R., Bregni, M., Erikson, J., Patterson, D., Gallo, R.C., and Croce, C.M. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:7824–7827.
Tsujimoto, Y., Gorham, J., Cossman, J., Jaffe, E., and Croce, C.M. (1985).Science 229:1390–1393.
Yoshida M.C., Sasaki M., Semba K., Yamanashi Y., Nishizawa M., Sukegawa J., Yamamoto T., Toyoshima K. (1985).Cytogenet. Cell Genet. 40:786 (abstr.).
Hayday, A.C., Gillies, S.D., Saito, H., Wood, C., Wiman, K., Hayward, W.S., and Tonegawa, S. (1984).Nature 307:334–340.
Lalley, P.A., and McKusick, V.A. (1985). Human Gene Map** 8.Cytogenet. Cell Genet. 40:536–566.
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Fujii, D., Brissenden, J.E., Derynck, R. et al. Transforming growth factor β gene maps to human chromosome 19 long arm and to mouse chromosome 7. Somat Cell Mol Genet 12, 281–288 (1986). https://doi.org/10.1007/BF01570787
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DOI: https://doi.org/10.1007/BF01570787