Log in

Induced and normal crystalline inclusions in plastids revealed by freeze-fracturing

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Aggregations of approximately 11 nm spaced spherical particles are described in both freeze-fractured and chemically fixed bean and spinach leaf plastids. It is suggested that the aggregations were induced as a result of the dehydrating action of the cryoprotectants used. It is tentatively concluded, on the basis of previous reports, that the aggregations consist of Fraction I protein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Akazawa, T., 1970: The structure and function of Fraction I protein. Progress in Phytochemistry2, 107–141.

    Google Scholar 

  • Bartels, P. G., andT. E. Weier, 1967: Particle aggregations in proplastids ofTriticum vulgare L. seedlings. J. Cell Biol.33, 243–253.

    PubMed  Google Scholar 

  • Bullivant, S., andA. Ames, 1966: A simple freeze-fracture replication method for electron microscopy. J. Cell Biol.29, 435–447.

    PubMed  Google Scholar 

  • De Greef, J. A., andJ. P. Verbelen, 1973: Physiological, stress and crystallites in leaf plastids ofPhaseolus vulgaris L. Ann. Bot.37, 593–596.

    Google Scholar 

  • Giesbrecht, P., 1968: Zur Darstellung der DNS von Bakterien und plastischer biologischer Strukturen mit Hilfe der Gefrierätzung. Zbl. f. Bakt.207, 198–221.

    Google Scholar 

  • Gunning, B. E. S., 1965: The greening process in plastids. 1. The structure of the prolamellar body. Protoplasma60, 111–130.

    Google Scholar 

  • —,M. W. Steer, andM. P. Cochrane, 1968: Occurrence, molecular structure, and induced formation of the “Stromacentre” in plastids. J. Cell Sci.3, 445–456.

    Google Scholar 

  • Kahn, A., 1968: Developmental physiology of bean leaf plastids. II. Negative contrast electron microscopy of tubular membranes in prolamellar bodies. Plant Physiol.43, 1769–1780.

    Google Scholar 

  • Kawashima, N., andS. G. Wildman, 1970: Fraction I protein. Ann. Rev. Plant Physiol.21, 325–358.

    Google Scholar 

  • Larsson, C., C. Collin, andP. A. Albertsson, 1973: The fine structure of chloroplast stroma crystals. J. Ultrastruct. Res.45, 50–58.

    PubMed  Google Scholar 

  • Lee, R. E., andA. Thompson, 1973: The stromacentre of plastids ofKalanchoë pinnata Persoon. J. Ultrastruct. Res.42, 451–456.

    PubMed  Google Scholar 

  • McIntyre, J. A., N. B. Gilula, andM. J. Karnovsky, 1974: Cryoprotectant—induced redistribution of intramembranous particles in mouse lymphocytes. J. Cell Biol.60, 192–203.

    PubMed  Google Scholar 

  • Nash, T., 1966: Chemical constitution and physical properties of compounds able to protect living cells against damage due to freezing and thawing. In: Cryobiology, pp. 179–211 (H. T. Meryman, ed.). London-New York: Academic Press.

    Google Scholar 

  • Ragetli, H. W. J., M. Weintraub, andE. Lo, 1970: Degeneration of leaf cells resulting from starvation after excision. I. Electron microscopic observations. Can. J. Bot.48, 1913–1922.

    Google Scholar 

  • Wrischer, M., 1973: Protein crystalloids in the stroma of bean plastids. Protoplasma77, 141–150.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pyliotis, N.A., Goodchild, D.J. Induced and normal crystalline inclusions in plastids revealed by freeze-fracturing. Protoplasma 85, 277–283 (1975). https://doi.org/10.1007/BF01567952

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01567952

Keywords

Navigation