Abstract
Exposure of Chinese hamster ovary (CHO) cells clone K 1BE4 to ultraviolet (UV) light at doses up to 86 ergs/mm2 did not significantly reduce cell survival, but UV doses of 86–648 ergs/mm2 produced an exponential cell killing. Observed mutation frequency to 6-thioguannine resistance induced by UV increases approximately in proportion to increasing doses up to 260 ergs/mm2 in a range of 5–648 ergs/mm2 examined. The pooled data of mutation frequency f(X) as a function of dose X from 0–260 ergs/mm2 is adequately described by f(X)=10−6 (13.6+2.04 X). That the UVinduced mutations to 6-thioguanine resistance affects the hypoxanthineguanine phosphoribosyltransferase (HGPRT) locus is supported by the observation that all randomly isolated drugresistant colonies contained highly reduced or undetectable HGPRT activity.
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Research supported by the Energy Research and Development Administration under contract with the Union Carbide Corporation.
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Hsie, A.W., Brimer, P.A., Mitchell, T.J. et al. The dose-response relationship for ultraviolet-light-induced mutations at the hypoxanthine-guanine phosphoribosyltransferase locus in Chinese hamster ovary cells. Somat Cell Mol Genet 1, 383–389 (1975). https://doi.org/10.1007/BF01538669
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DOI: https://doi.org/10.1007/BF01538669