Summary
The formation of the tapetal periplasmodium inTradescantia bracteata is described at the electron microscope level and methods are given for the preparation of material. Evidence, both fine-structural and cytochemical, is presented in support of the view that the tapetal periplasmodium inTradescantia posesses an organized, functional ultrastructure. The changes which occur after dissolution of the tapetal walls appear to be a reorganization rather than a degeneration. The observation that tapetal membranes penetrate the exine during the later stages of pollen development suggests that a physical association may develop between the cytoplasm of the male gametophyte and that of the sporophyte.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arens, K., 1949: Prova de calose por meio da microscopia a luz fluorescente e aplicoes do metodo. Lilloa18, 71–75.
Baker, J. R., 1946: The histochemical recognition of lipine. Quart. J. Microscop. Sci.87, 441–471.
Barka, T., andP. J. Anderson, 1963: Histochemistry. Theory, practice, and bibliography. New York: Harper and Row.
Deitch, D. A., 1955: Microspectrophotometric studies of the binding of the anionic dye, naphthol yellow S, by tissue sections and by purified proteins. Lab. Invest.4, 324–351.
Echlin, P., andH. Godwin, 1968 a: The ultrastructure and ontogeny of pollen inHelleborus foetidus L. I. The development of the tapetum and Ubisch bodies. J. Cell Sci.3, 161–174.
— —, 1968 b: The ultrastructure and ontogeny of pollen inHelleborus foetidus L. II. Pollen grain development through the callose special wall stage. J. Cell Sci.3, 175–186.
— —,B. Chapman, andR. Angold, 1966: The structure of polyvesicular bodies associated with cell walls in develo** anthers ofIpomoea purpurea (L.) Roth. VIth Int. Congr. Electron Microsc., Kyoto, vol. II, 317–318.
Heslop-Harrison, J., 1963: Ultrastructural aspects of differentiation in sporogenous tissue. Symp. Soc. exp. Biol.17, 315–340.
—, 1964: Cell walls, cell membranes, and protoplasmic connections during meiosis and pollen development. In: Pollen physiology and fertilization, ed.Linskens, Amsterdam: North Holland.
—, 1966: Cytoplasmic connections between angiosperm meiocytes. Ann. Bot.30, 221–230.
—, 1968: Wall development within the microspore tetrad ofLilium longiflorum. Canad. J. Bot.46, 1185–1191.
Jensen, W. A., 1962: Botanical histochemistry. London: Freeman.
La Cour, L. F., J. Chayen, andP. S. Gahan, 1958: Evidence for lipid material in chromosomes. Exp. Cell Res.14, 469–485.
Lane, G. R., andE. P. Evans, 1966: X-ray induction of chromosome aberrations in relation to nuclear volume inTradescantia bracteata. In: Chromosomes today, vol. I, ed.Darlington andLewis, Edinburgh: Oliver and Boyd.
Mepham, R. H., andG. R. Lane, 1968: Exine and the role of the tapetum in pollen development. Nature219, 961–962.
— —, 1969 a: Role of the tapetum in the development ofTradescantia pollen. Nature221, 282–284.
— —, 1969 b: Nucleopores and polyribosome formation. Nature221, 288–289.
Nachlas, M. M., K.-C. Tsow, E. De Souza, C. S. Cheng, andA. M. Seligman, 1957: Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J. Histochem. Cytochem.5, 420–436.
Pearse, A. G. E., 1960: Histochemistry. Theoretical and applied, 2nd ed., London: Churchill.
Reeve, R. M., 1959: A specific hydroxylamine-ferric chloride reaction for the histochemical localization of pectin. Stain Technol.34, 209–211.
Rowley, J. R., K. Mühlethaler, andA. Frey-Wyssling, 1959: A route for the transfer of materials through the pollen grain wall. J. Biophys. Biochem. Cytol.6, 537–538.
Skvarla, J. J., andD. A. Larsen, 1966: Fine structural studies of Zeamays pollen. I. Cell membranes and exine ontogeny. Amer. J. Bot.53, 1112–1125.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mepham, R.H., Lane, G.R. Formation and development of the tapetal periplasmodium inTradescantia bracteata . Protoplasma 68, 175–192 (1969). https://doi.org/10.1007/BF01247903
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01247903