Abstract
Amyloid A (AA) protein is derived from serum amyloid A (SAA) and deposited as beta-pleated sheet fibrils in reactive amyloidosis, a disease that occurs spontaneously in golden Syrian hamsters. The precursor SAA is an acute-phase reactant in many species including hamsters, and in this report we have defined the in vivo kinetic and dosage responses for SAA mRNA accumulation in hamsters following administration of various cytokines. Elevations in levels of hepatic SAA mRNA were documented when the doses of interleukin-1, interleukin-6, and tumor necrosis factor were increased. The increase in dosages applied ranged from 2 1/2-fold for interleukin-6 to 10-fold for interleukin-1. SAA transcipt levels were highest 8 h following administration of interleukin-6 or tumor necrosis factor, whereas maximal amounts of SAA-specific mRNA were found 24 h after administration of interleukin-1.
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References
Kushner, I., M. K. Ganapathi, andS. S. Macintyre. 1989. Regulation of biosynthesis and secretion of human C-reactive protein and serum amyloid A.In Acute Phase Proteins in the Acute Phase Response. M. B. Pepys, editor. Springer-Verlag, London. 69–83.
Meek, R. L., andE. P. Benditt. 1986. Amyloid A gene family expression in different mouse tissues.J. Exp. Med. 164:2006–2017.
Woo, P., J. D. Sipe, C. A. Dinarello, andH. R. Colten. 1987. Structure of a human serum amyloid A gene and modulation of its expression in transfected L cells.J. Biol. Chem. 262:15790–15795.
Ramadori, G., J. D. Sipe, andH. R. CoLten. 1985a. Expression and regulation of the murine serum amyloid A (SAA) gene in extraheptic sites.J. Immunol. 135:3645–3647.
Ramadori, G., J. D. Sipe, C. A. Dinarello, S. B. Mizel, andH. R. Golten. 1985b. Pretranslational modulation of acute phase hepatic protein synthesis by murine recombinant interleukin-1 (IL-1) and purified human IL-1.J. Exp. Med. 162:930–942.
Webb, C. F., P. W. Tucker, andS. B. Dowton. 1989. Expression and sequence analyses of serum amyloid A in the Syrian hamster.Biochemistry 28:4785–4790.
Husebekk, A., B. Skogen, G. Husby, andG. Marhaug. 1985. Transformation of amyloid precursor SAA to protein AA and incorporation in amyloid fibrils in vivo.Scand. J. Immunol. 21:283–287.
Coe, J. E., andM. J. Ross. 1990. Amyloidosis and female protein in the Syrian hamster. Concurrent regulation by sex hormones.J. Exp. Med. 171:1257–1267.
Sambrook, J., E. F. Fritsch, andT. Maniatis. 1989. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Dowton, S. B., and S. N.Holden. 1991. C-reactive protein (CRP) of the Syrian hamster (Unpublished).
Schwartz, R. J., J. A. Haron, K. N. Rothblum, andA. Dugaiczyk. 1980. Regulation of muscle differentiation: Cloning of sequences from a-actin messenger ribonucleic acid.Biochemistry 79:5883–5890.
Brissette, L., I. Young, S. Narindrasorasak, R. Kisilevsky, andR. Deeley. 1989. Differential induction of the serum amyloid A gene family in response to an inflammatory agent and to amyloid-enhancing factor.J. Biol. Chem. 264:19327–19332.
Selinger, M. J., K. P. W. J. McAdam, M. M. Kaplan, J. D. Sipe, S. N. Vogel, andD. L. Rosenstreich. 1980. Monokine-induced synthesis of serum amyloid A protein by hepatocytes.Nature 285:498–500.
Woo, P., J. Sipe, C. A. Dinarello, andH. R. Colten. 1987. Structure of a human serum amyloid A gene and modulation of its expression in transfected L cells.J. Biol. Chem. 262:15790–15795.
Moshage, H. J., H. M. J. Roelofs, andJ. F. Van Pelt. 1988. The effect of interleukin-1, interleukin-6 and its interrelationship on the synthesis of serum amyloid A and C-reactive protein in primary cultures of adult human hepatocytes.Biochem. Biophys. Res. Commun. 155:112–117.
Castell, J. V., M. J. Gomez-Lechon, M. David, T. Hirano, T. Kishimoto, andP. C. Heinrich. 1988. Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in huamn hepatocytes.FEBS Lett. 232:347–350.
Brinckerhoff, C. E., T. I. Mitchell, M. J. Karmilowicz, B. Kluve-Beckerman, andM. D. Benson. 1989. Autocrine induction of collagenase by serum amyloid A-like and B2-microglobulin-like proteins.Science 243:655–657.
Benson, M. D., M. A. Aldo-Benson, T. Shirahama, Y. Borel, andA. S. Cohen. 1975. Suppression of in vitro antibody response by a serum factor (SAA) in experimentally induced amyloidosis.J. Exp. Med. 142:236–241.
Tobias, P. S., K. P. W. J. MacAdam, andR. J. Ulevitch. 1982. Interaction of bacterial lipopolysaccharide with acute-phase rabbit serum and isolation of two forms of rabbit serum amyloid A.J. Immunol. 128:1420.
Hol, P. R., andE. Gruys. 1984. Amyloid A proteins in different species.Appl. Pathol. 2:316–327.
Husby, G., A. Husebekk, B. Skogen, K. Sletten, G. Marhaug, J. Magnus, andV. Syversen. 1988. Serum amyloid A (SAA) — the precursor of protein AA in secondary amyloidosis. In Proceedings of the 4th International Colloquium on Atherosclerosis. C. L. Malmendier, editor. Brussels, Belgium.
Marhaug, G., G. Husby, andS. B. Dowton. 1990. Mink serum amyloid A protein. Expression and primary structure based on cDNA sequences.J. Biol. Chem. 265:10049–10054.
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Dowton, S.B., Peters, C.N. & Jestus, J.J. Regulation of serum amyloid a gene expression in Syrian hamsters by cytokines. Inflammation 15, 391–397 (1991). https://doi.org/10.1007/BF00917355
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DOI: https://doi.org/10.1007/BF00917355