Summary
A method for preparative isolation of serum lipoproteins by a combination of differential and density gradient ultracentrifugation is presented. Total plasma lipoproteins are first isolated in a concentrated form by ultracentrifugation in a fixed angle rotor at a plasma background density of 1.21 kg/l. Subsequently, the various lipoprotein classes are separated by density gradient ultracentrifugation in a swinging bucket rotor. The procedure requires only two ultracentrifugation steps and combines advantages of both ultracentrifugation techniques.
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Abbreviations
- VLDL:
-
very low density lipoproteins
- LDL:
-
low density lipoproteins
- HDL:
-
high density lipoproteins
- VHDL:
-
very high density lipoproteins
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Terpstra, A.H.M., Pels, A.E. Isolation of plasma lipoproteins by a combination of differential and density gradient ultracentrifugation. Z. Anal. Chem. 330, 149–151 (1988). https://doi.org/10.1007/BF00470729
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DOI: https://doi.org/10.1007/BF00470729