Abstract
The absence of soy phosphatidylcholine (PC) in purified diets fed to juvenile lobstrs causes a significant decrease in the concentrations of total cholesterol and phospholipids in the serum. When egg PC, bovine PC, phosphatidylinositol, and ovine cephalin are used as substitutes for the soy PC, cholesterol and phospholipid levels in the serum are also significantly reduced. Cholesterol and phospholipid concentrations in the serum are highly correlated. Analysis of levels of hepatopancreatic cholesterol indicate that intestinal absorption of cholesterol is not impaired by the absence of dietary PC or by the presence of a phospholipid substitute. We suggest that the PC molecule is an important component of a lipoprotein complex that effectively transfers cholesterol, an essential nutrient for lobsters, from the hepatopancreas to the hemolymph.
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Literature Cited
Aiken, D. E.: Photoperiod, endocrinology, and the crustacean molt cycle. Science, N. Y. 164, 149–155 (1969)
Aiken, D. E.: Procecdysis, setal development, and molt prediction in the american lobster, (Homarus americanus). J. Fish. Res. Bd Can. 30, 1337–1344 (1973)
ando, T., A. Kanazawa, S. Teshima, J. Patrois and H. J. Ceccaldi: Variation in the lipids of tissues during the molt cycle of the prawn. Bull. Jpn Soc. Sci. Fish 43(12), 1445–1449 (1977)
Blaton, V., B. DeClercq, D. Vandamme, F. Soetewey and H. Peeters: The human plasma lipids and lipoproteins under influence of EPL-therapy. In: Phosphatidylcholine, pp 125–132. Ed. by H. Peeters. New York: Springer Verlag 1976
Bligh, E. G. and M. A. Scott: Blood lipids of the lobster, Homarus americanus. J. Fish. Res. Bd Can. 23, 1629–1631 (1966)
Bowser, P. R. and R. Rosemark: Mortalities of cultured lobsters, Homarus, associated with a molt death syndrome. Aquaculture 23, 11–18 (1981)
Castell, J. D., E. G. Mason and J. F. Covey: Cholesterol requirements of juvenile American lobsters. J. Fish. Res. Bd Can. 32, 1431–1435 (1975)
Courchaine, A. J., W. H. Miller and D. B. Stein, Jr.: Rapid semimicro procedure for estimating free and total cholesterol. Clin. Chem. 5, 609–614 (1959)
Conklin, D. E., K. Devers and C. E. Bordner: Development of artificial diets for the lobster, Homarus americanus. Proc. World Mariculture Soc. 8, 841–852 (1977)
Conklin, D. E., L. R. D'Abramo, C. E. Bordner and N. A. Baum: A successful purified diet for the culture of juvenile lobsters: the effect of lecithin. Aquaculture 21, 243–249 (1980)
D'Abramo, L. R., C. E. Bordner, D. E. Conklin, G. R. Daggett and N. A. Baum: Relationships among dietary lipids, tissue lipids, and growth in juvenile lobsters. Proc. World Mariculture Soc. 11, 335–345 (1980)
D'Abramo, L. R., C. E. Bordner, D. E. Conklin and N. A. Baum: Essentiality of dietary phosphatidylcholine for the survival of juvenile lobsters. J. Nutrition 111, 63–69 (1981)
Dwivedy, A. K. and R. G. Bridges: The effect of dietary changes on the phospholipid composition of the hemolymph lipoproteins proteins of larvae of the housefly, Musca domestica. J. Inset Physiol. 19, 559–576 (1973)
Ewing, R. D. and F. J. Finamore: Phospholipid metabolism during development of the brine shrimp Artemia salina II. Snythesis of phosphatidylcholine by a microsomal enzyme system from nauplii. Biochim. biophys. Acta 218, 474–481 (1970)
Gagosian, R. B., R. A. Bourbonniere, W. B. Smith, E. F. Couch, C. Blanton and W. Novak: Lobster molting hormones: isolation and biosynthesis of edcysterone. Experientia 30(7), 723–724 (1974)
Gilbert, L. I. and J. D. O'Connor: Lipid metabolism and transport in Arthropods. In: Chemical zoology, Vol. 5, pp 229–253. Ed. by M. Florkin and B. T. Sheer, New York: Academic Press 1970
Hirsch, M. J., J. H. Growdon and R. H. Wortman: Relations between dietary choline or lecithin intake, serum choline levels, and various metabolic indices. Metabolism 27(8), 953–960 (1978)
Kanazawa, A. and S. Teshima: In vivo conversion of cholesterol to steroid hormones in the spiny lobster, Panulirus japonica. Bull. Jpn Soc. Sci. Fish. 37, 891–898 (1971)
Kanzawa, A., S. Teshima, S. Tokiwa, M. Endo and F. A. A. Razek: Effects of short-necked clam phospholipids on the growth of the prawn. Bull. Jpn Soc. Sci. Fish. 45, 961–965 (1979)
Lester, R., M. C. Carey, L. A. Cooperstein and S. R. Dowd: Crustacean intestinal detergent promotes sterol solubilization. Science, N. Y. 189, 1098–1100 (1975)
Lee, R. F. and D. L. Puppione: Serum lipoproteins in the spiny lobster, Panulirus interruptus. Comp. Biochem. Physiol. 59B, 239–243 (1978)
Mankura, M., D. Dalimunthe and M. Kayama: Comparative biochemical studies on plasma cholesterol — II. Bull. Jpn Soc. Sci. Fish. 46(5), 583–586 (1980)
O'Connor, J. D. and L. I. Gilbert: Alterations in lipid metabolism associated with premolt activity in a land crab and freshwater crayfish. Comp. Biochem. Physiol. 29, 889–904 (1969)
Peeters, H.: The biological significance of plasma phospholipids. In: Phosphatidylcholine, pp 10–33. Ed. by H. Peeters. New York: Springer Verlag 1976
Sandhu, R. S.: Serum phospholipids determined without acid digestion. Clin. Chem. 22(12), 1973–1975 (1976)
Sgoutas, D. S.: Fatty acid specificity of plasma phosphatidylcholine: cholesterol acyltransferase. Biochem. 11(2), 293–296 (1972)
Shieh, M. S.: The biosynthesis of phospholipids in the lobster, Homarus americanus. Comp. Biochem. Physiol. 30, 679–684 (1969)
Teshima, S. and A. Kanazawa: Release and transport of lipids in the prawn. Bull. Jpn Soc. Sci. Fish. 44(11), 1269–1274 (1978)
Teshima, S. and A. Kanazawa: Lipid transport mechanism in the prawn. Bull. Jpn Soc. Sci. Fish. 45(10), 1341–1346 (1979)
Teshima, S. and A. Kanazawa: Transport of dietary lipids and role of serum lipoproteins in prawn. Bull. Jpn Soc. Sci. Fish 46(1), 51–55 (1980a)
Teshima, S. and A. Kanazawa: Lipid constituents of serum lipoproteins in the prawn. Bull. Jpn Soc. Sci. Fish 46(1), 57–62 (1980b)
Turunen, S. and T. Kastari: Digestion and absorption of lecithin in larvae of the cabbage butterfly, Pieris brassicae. Comp. Biochem. Physiol. 62A, 933–937 (1979)
Zlatkis, A., B. Zak and A. J. Boyle: A new method for the direct determination of serum cholesterol. J. Lab. Clin. Med. 41, 486–492 (1963)
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Communicated by J. M. Lawrence, Tampa
This work is a result of research sponsored in part by NOAA, Office of Sea Grant, Department of Commerce, under Grant No. 04-8-MO1-189 R/A 28. The US Government is authorized to produce and distribute reprints for governmental purposes notwithstanding any copyright notation that may appear hereon
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D'Abramo, L.R., Bordner, C.E. & Conklin, D.E. Relationship between dietary phosphatidylcholine and serum cholesterol in the lobster Homarus sp.. Marine Biology 67, 231–235 (1982). https://doi.org/10.1007/BF00401289
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DOI: https://doi.org/10.1007/BF00401289