Summary
The phage 11 of R. meliloti performs generalized transduction. This was confirmed by the variety of single markers transferred and by separating transducing particles containing BUdR-labelled bacterial DNA. The transduction frequencies depended on the marker. Linked alleles were mapped by cotransduction on fragments of bacterial DNA equal in size to the phage DNA. With crosses between antibiotic resistancy and auxotrophic markers a partial map was constructed with str, cml, pur-19, and leu-44 sites. With a few multi-auxotrophic mutants linkage data of conjugation were compared with the linkage by cotransduction.
Similar content being viewed by others
References
Backhaus, H., Schmieger, H.: The origin of the DNA in a special class of generalized transducing particles of Salmonella-phage P22. Mol. Gen. Genet. 131, 123–135 (1974)
Barabás, I., Sik, T.: Phenotypic reversion of nitrogenase in pleiotropic mutants of Rhizobium meliloti. Can. J. Microbiol. 25, 298–301 (1979)
Buchanan-Wollaston, V.: Generalized Transduction in Rhizobium leguminosarum. J. Gen. Microbiol. 112, 135–142 (1979)
Casadesus, J., Olivares, J.: Rough and Fine Linkage Map** of the Rhizobium meliloti Chromosome. Mol. Gen. Genet. 174, 203–209 (1979)
Dallmann, G., Orosz, L., Sain, B.: Restriction Map** of DNA of Temperate Rhizobium meliloti Phage 16-3 Comparison of Genetic and Physical Maps Indicates a Long Genetically Silent Chromosomal Area. Mol. Gen. Genet. 176, 439–448 (1979)
Ebel-Tsipis, J., Botstein, D., Fox, M.S.: Generalized Transduction by Phage P22 in Salmonella typhimurium. I. Molecular Origin of Transducing DNA. J. Mol. Biol. 71, 433–448 (1972)
Ikeda, H., Tomizawa, J.-I.: Transducing Fragments in Generalized Transduction by P1. I. Molecular Origin of the Fragments. J. Mol. Biol. 14, 85–109 (1965)
Kondorosi, A., Barabás, I., Sváb, Z., Orosz, L., Sik, T., Hotchkiss, R.D.: Evidence for Common Genetic Determinants of Nitrogenase and Nitrate Reductase in Rhizobium meliloti. Nature (London) New Biol. 246, 153–154 (1973)
Kondorosi, A., Kiss, G.B., Forrai, T., Vincze, E., Bánfalvi, Z.: Circular linkage map of Rhizobium meliloti chromosome. Nature 268, 525–527 (1977)
Kowalski, M.: Transduction in Rhizobium meliloti. Acta Microbiol. Pol. 16, 7–12 (1967)
Kowalski, M.: Transducing phages of Rhizobium meliloti. Acta Microbiol. Bol. 2, 109–113 (1970)
Kowalski, M.: Transduction of effectiveness in Rhizobium meliloti in symbiotic nitrogen fixation in plants (P.S. Nutman, ed.), pp. 63–67. London: Cambridge University Press 1974
Orosz, L., Sváb, Z., Kondorosi, A., Sik, T.: Genetic studies on Rhizobiophage 16-3. I. Genes and Functions on the chromosome. Mol. Gen. Genet. 125, 341–350 (1973)
Schmieger, H.: Die molekulare Struktur transduzierender Partikel beim Salmonella-Phagen P22. I. Dichtegradienten-Untersuchungen an intakten Phagen. Mol. Gen. Genet. 102, 336–347 (1968)
Sik, T., Orosz, L.: Chemistry and genetics of Rhizobium meliloti phage 16-3. Plant and Soil, Spec. Vol. 57–62 (1971)
Sik, T., Horváth, J., Misra, A.K., Chattopadhyay, S.: General transduction in Rhizobium meliloti. Acta Microbiol. Acad. Sci. Hung. 25, 129 (1978)
Sváb, Z., Kondorosi, A., Orosz, L.: Specialized Transduction of a Cysteine Marker by Rhizobium meliloti Phage 16-3. J. Gen. Microbiol. 106, 321–327 (1978)
Szende, K., Ördögh, F.: Die Lysogenie von Rhizobium meliloti. Naturwissenschaften 47, 404–405 (1960)
Wu, T.T.: A Model for Three-Point Analysis of Random General Transduction. Genetics 54, 405–410 (1966)
Zinder, N.D.: Bacterial Transduction. J. Cell. Comp. Physiol. 45, (suppl. 2) 23 (1955)
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Rights and permissions
About this article
Cite this article
Sik, T., Horváth, J. & Chatterjee, S. Generalized transduction in Rhizobium meliloti . Molec. gen. Genet. 178, 511–516 (1980). https://doi.org/10.1007/BF00337855
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00337855