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Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyoty** and hybridization with cloned genes

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Abstract

Significant differences in electrophoretic karyoty** patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains showed unique karyotypes. Chromosomal DNAs of different strains were hybridized with cloned genes of Y. lipolytica (URA3, LEU2, ARS18 and ARS68), which revealed four different bands in most strains. We conclude that the haploid chromosome number of Y. lipolytica is at least four, and possibly five or six. Electrophoretic karyoty** and hybridization with cloned genes of Y. lipolytica provided evidence of a large divergence between Y. lipolytica and related species of Saccharomycopsis, Endomycopsella and Endomyces.

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Communicated by K. Esser

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Naumova, E., Naumov, G., Fournier, P. et al. Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyoty** and hybridization with cloned genes. Curr Genet 23, 450–454 (1993). https://doi.org/10.1007/BF00312633

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  • DOI: https://doi.org/10.1007/BF00312633

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