Abstract
Significant differences in electrophoretic karyoty** patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains showed unique karyotypes. Chromosomal DNAs of different strains were hybridized with cloned genes of Y. lipolytica (URA3, LEU2, ARS18 and ARS68), which revealed four different bands in most strains. We conclude that the haploid chromosome number of Y. lipolytica is at least four, and possibly five or six. Electrophoretic karyoty** and hybridization with cloned genes of Y. lipolytica provided evidence of a large divergence between Y. lipolytica and related species of Saccharomycopsis, Endomycopsella and Endomyces.
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Asakura K, Iwaguichi SI, Homma M, Sukai T, Higashide K, Tanaka K (1991) J Gen Microbiol 137:2531–2538
Barns SM, Lane DJ, Sogin ML, Ribeau C, Weisburg WG (1991) J Bacteriol 173:2250–2255
Buckholz RG, Gleeson MAG (1991) Bio/Technol 9: 1067–1072
Davidow L, de Zeeuw JR (1985) European Patent EPO138508
Esser K, Stahl U (1976) Mol Gen Genet 146:101–106
Fournier F, Guyaneux L, Chasles M, Gaillardin C (1991) Yeast 7:25–36
Gaillardin CM, Ribet A-M (1987) Curr Genet 11:369–375
Gaillardin CM, Charoy V, Heslot H (1973) Arch Microbiol 92:69–83
Iwaguichi SI, Homma M, Tanaka K (1990) J Gen Microbiol 136:2433–2442
Johnston JR, Contopoulou CP, Mortimer RK (1988) Yeast 4:191–198
Kurischko C (1986) J Basic Microbiol 1:33–41
Kreger van Rij NJW, Veenhuis M (1973) Ant van Leeuwenhoek 39:481–490
Magee BB, Koltin Y, Gorman JA, Magee PT (1988) Mol Cel Biol 8:4721–4726
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Naumov GI, Naumova ES, Lantto RA, Louis EJ, Korhola M (1992) Yeast 8:599–612
Nga BH, Abu Bakar FD, Loh GH, Chiu LL, Harashima S, Oshima Y, Heslot H (1992) J Gen Microbiol 138:225–227
Ogrydziak D (1988) J Basic Microbiol 28:185–196
Ogrydziak D, Bassel J, Contopoulou R, Mortimer R (1978) Mol Gen Genet 163:229–239
Ogrydziak D, Bassel J, Mortimer R (1982) Mol Gen Genet 188:179–183
Phaff HJ (1985) In: Demain AL, Solomon NA (eds) Biology of industrial microorganisms. Benjamin/Cummings, London, pp 537–562
Rikkerink EHA, Magee BB, Magee PT (1990) Infect Immun 58:949–954
Sor F, Fukuhara H (1989) Yeast 5:1–10
Steensma HY, de Jongh FCM, Linnekamp M (1988) Curr Genet 14:311–317
Walt JP van der, von Arx JA (1980) Ant van Leeuwenhoek 46:517–521
Wickerham LJ, Kurtzman CP, Herman AI (1970) Spectrum Monogr Ser Arts Sci Georgia 1:81–92
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Naumova, E., Naumov, G., Fournier, P. et al. Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyoty** and hybridization with cloned genes. Curr Genet 23, 450–454 (1993). https://doi.org/10.1007/BF00312633
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DOI: https://doi.org/10.1007/BF00312633