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Biochemical differences between embryogenic and nonembryogenic callus of Picea abies (L.) Karst

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Abstract

Both embryogenic and nonembryogenic calli of Picea abies (L.) Karst. were initiated from the hypocotyl region of immature embryos. The two callus phenotypes were manually separated and subsequently maintained independently, but under identical culture conditions. Biochemical analysis of the two phenotypes revealed significant differences in ethylene evolution rate and in concentrations of glutathione and total reductants. Due to the constancy of the genetic background, age and growth conditions of the two callus types, differences in the measured quantities are not likely to be traceable to the genetic origin of the callus and serve to highlight biochemical changes associated with somatic embryogenesis in Norway spruce.

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Abbreviations

GSH:

glutathione

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

N6-benzyl adenine

E:

embryogenic

NE:

nonembryogenic

NS:

Norway spruce

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Authors and Affiliations

  1. Forest Biology Division, The Institute of Paper Chemistry, P.O. Box 1039, 54912, Appleton, WI, USA

    S. R. Wann, M. A. Johnson, T. L. Noland & J. A. Carlson

Authors
  1. S. R. Wann
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  2. M. A. Johnson
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  3. T. L. Noland
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  4. J. A. Carlson
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Communicated by J. M. Widholm

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Wann, S.R., Johnson, M.A., Noland, T.L. et al. Biochemical differences between embryogenic and nonembryogenic callus of Picea abies (L.) Karst. Plant Cell Reports 6, 39–42 (1987). https://doi.org/10.1007/BF00269735

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  • DOI: https://doi.org/10.1007/BF00269735

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