Abstract
Suspension cultures of Berberis wilsonae produce 4 berberine-type alkaloids: berberine, palmatine, columbamine and jatrorrhizine. In particular the formation of the phenolic alkaloids columbamine and jatrorrhizine and of berberine proves to be dependent on the concentration of dissolved oxygen. With higher aeration rates, berberine and jatrorrhizine yields can be increased considerably. Thus we reached an alkaloid yield of more than 3 g × 1−1 with 50% dissolved oxygen tension in the medium. As far as we know this is one of the best results in fermenting of alkaloid-producing cell cultures.
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Abbreviations
- pO2 :
-
dissolved oxygen concentration in % saturation (using air)
- HPLC:
-
high-performance liquid chromatography
- vvm:
-
volume air × volume medium−1 × minute−1
- rpm:
-
revolutions per minute
- IAA:
-
indole-3-acetic acid
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
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Communicated by W. Barz
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Breuling, M., Alfermann, A.W. & Reinhard, E. Cultivation of cell cultures of Berberis wilsonae in 20-l airlift bioreactors. Plant Cell Reports 4, 220–223 (1985). https://doi.org/10.1007/BF00269294
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DOI: https://doi.org/10.1007/BF00269294