Summary
The cellulase activities of six thermophilic fungi were compared. Although the thermophilic fungi grew at relatively high temperatures (>45°C) the optimum temperatures for assaying the various cellulase activities were only slightly higher than the optimum temperatures for the mesophilic fungi, Trichoderma harzianum. Over prolonged incubation (> 24 h) the thermophilic strains demonstrated a higher hydrolytic potential as a result of the greater thermostability of the cellulase components. Although the extracellular cellulase activities had similar pH and temperature optima, in some cases the thermostability of the extracellular components were considerably lower.
Similar content being viewed by others
References
Araujo EF, Barros EG, Caldas RA, Silva DO (1983) Beta-glucosidase activity of a thermophilic cellulolytic fungus, Humicola sp. Biotech Lett 5:781–784
Breuil C, Saddler JN (1985) Comparison of the 3,5-dinitrosalicylic acid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity. Enzyme Microbial Technol 7:327–331
Breuil C, Wojtczak G, Saddler JN (1986) Production and localization of cellulases and β-glucosidase from the thermophilic fungus Thielavia terrestris. Biotech Lett 8:673–676
Cooney DG, Emerson A (1964) In: Freeman WH (ed) Thermophilic fungi. An account of their biology, activities and classification. Publ. Co., San Francisco 188 pp
Durand H, Soucaille P, Tiraby G (1984) Comparative study of cellulases and hemicellulases from 4 fungi: mesophiles Trichoderma reesei and Penicillium sp. and thermophiles Thielavia terrestris and Sporotrichum cellulophilum. Enzyme Microbial Technol 6:175–180
Jain MK, Kapoor KK, Mishera MM (1979) Cellulase activity degradation of cellulose and lignin and humus formation by thermophilic fungi. Trans Br Mycol Soc 73:85–89
Komura I, Awao T, Yamada K (1978) Thermostable cellulase and method for producing the same U.S. Patent 4 106 989
Ladisch MR, Lin KW, Voloch M, Tsao GT (1983) Process considerations in the enzymatic hydrolysis of biomass. Enzyme Microbial Technol 5:82–102
Mandels M, Weber J, Parizek R (1971) Enhanced cellulase production by a mutant of Trichoderma viride. Appl Microbiol 21:152–154
McHale A, Coughlan MP (1980) Synergistic hydrolysis of cellulose by components of the extracellular cellulase system of Talaromyces emersonii. FEBS Lett 117:319–322
Montenecourt BS, Eveleigh DE (1978) Antibiotic discs an improvement in the filter paper assay for cellulase. Biotech Bioeng 20:297–300
Reese ET (1975) Summtary statement on the enzyme system. In: Wilke CR (ed) Cellulose as a chemical and energy source. John Wiley and Sons Inc., New York, pp 77–80
Saddler JN, Hogan CM, Louis-Seize G (1985) A comparison between the cellulase systems of Trichoderma harzianum E58 and Trichoderma reesei C30. Appl Microbiol Biotechnol 22:139–145
Sen S, Abraham TK, Chakrabarty SL (1982) Characteristics of the cellulase produced by Myceliophthora thermophila. Can J Microbiol 28:271–277
Skinner WA, Tokuyama F (1978) Production of cellulase by a thermophilic fungus Thielavia terrestris. U.S. patent 4081 328
Somogyi M (1952) Notes on sugar determinations. J Biol Chem 195:19–23
Wood TM (1968) Cellulolytic enzyme system of Trichoderma koningii: separation of components attacking native cotton. Biochem J 109:217–227
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wojtczak, G., Breuil, C., Yamada, J. et al. A comparison of the thermostability of cellulases from various thermophilic fungi. Appl Microbiol Biotechnol 27, 82–87 (1987). https://doi.org/10.1007/BF00257258
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00257258