Summary
Enzymatic conversion of lignocellulosic material to fuels and chemicals depends on a initial pretreatment to render the cellulose more susceptible to enzymatic attack. Biological delignification of aspenwood with the fungus Phlebia tremellosus was compared to steaming as a pretreatment method.
The biologically delignified aspenwood (BDA) had a high pentosan content and did not contain inhibitors of enzymatic hydrolysis or subsequent fermentation. In contrast, the steamed aspenwood required a water extraction step to remove the inhibitory material and this step also removed most of the pentosan. The yield of treated material was 90% from biological delignification and 70% from steaming.
The cellulose in the BDA was less accessible to the cellulase enzymes than the steamed aspenwood. Combined hydrolysis and fermentation with Saccharomyces cerevisiae gave a lower yield of ethanol from BDA than from the steamed aspenwood, but the yields based on the weight of substrate before pretreatment were comparable. Combined hydrolysis and fermentation with Klebsiella pneumoniae gave higher yields of butanediol from BDA than from steamed aspenwood, because Klebsiella can ferment the xylose which was present in the biologically treated aspenwood. Trichoderma harzianum produced lower levels of cellulase enzymes when grown on BDA than when grown on steamed aspenwood and this was related to the xylan found in the biologically treated material.
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Abbreviations
- BDA:
-
biologically delignified aspenwood
- SEA-WI:
-
steam-exploded, water-extracted aspenwood
- AI-SEA-WI:
-
acid-impregnated, steam-exploded, water-extracted aspenwood
- CHF:
-
combined hydrolysis and fermentation
- FP:
-
filter paper
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Mes-Hartree, M., Yu, E.K.C., Reid, I.D. et al. Suitability of aspenwood biologically delignified with Pheblia tremellosus for fermentation to ethanol or butanediol. Appl Microbiol Biotechnol 26, 120–125 (1987). https://doi.org/10.1007/BF00253894
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DOI: https://doi.org/10.1007/BF00253894