Abstract
When grown in complex or synthetic media, Lactobacillus casei B 80 synthesizes a mitomycin C-inducible polypeptide with very specific bactericidal activity against the sensitive strain Lactobacillus casei B 109. The amount of secreted bacteriocin in the culture solution was low, about 1 mg/l. The bacteriocin which we called caseicin 80, was also detectable in cell extracts, although only 2% of the total activity was retained intracellularly. Caseicin 80 was concentrated by ultrafiltration and purified by cation exchange chromatography with Cellulose SE-23 and Superose. The molecular weight was in the range of M r=40,000–42,000 and the isoelectric point was pH 4.5.
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Rammelsberg, M., Müller, E. & Radler, F. Caseicin 80: purification and characterization of a new bacteriocin from Lactobacillus casei . Arch. Microbiol. 154, 249–252 (1990). https://doi.org/10.1007/BF00248963
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DOI: https://doi.org/10.1007/BF00248963