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Fermentation of L-arabinose, D-xylose and D-glucose by ethanologenic recombinant Klebsiella oxytoca strain P2

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Summary

Recombinant Klebsiella oxytoca strain P2 carrying genes for pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis was evaluated for its ability to ferment arabinose, xylose and glucose alone and in mixtures in pH-controlled batch fermentations. This organism produced 0.34–0.43 g ethanol/g sugar at pH 6.0 and 30°C on 8% sugar substrate and demonstrated a preference for glucose. Sugar utilization was glucose > arabinose > xylose and ethanol production was xylose > glucose > arabinose.

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Authors and Affiliations

  1. U. S. Department of Agriculture, Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 61604, Peoria, Illinois, USA

    Rodney J. Bothast, Badal C. Saha & A. Vance Flosenzier

  2. Department of Microbiology and Cell Science, University of Florida, 32611, Gainsville, Florida, USA

    Lonnie O. Ingram

Authors
  1. Rodney J. Bothast
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  2. Badal C. Saha
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  3. A. Vance Flosenzier
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  4. Lonnie O. Ingram
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Bothast, R.J., Saha, B.C., Flosenzier, A.V. et al. Fermentation of L-arabinose, D-xylose and D-glucose by ethanologenic recombinant Klebsiella oxytoca strain P2. Biotechnol Lett 16, 401–406 (1994). https://doi.org/10.1007/BF00245060

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