Abstract
Somatic embryogenesis and subsequent plant regeneration of Euonymus europaeus L (European Spindle Tree) were obtained from square pieces of mature zygotic embryos with an intervening callus phase. Callus and somatic embryos were induced using a Murashige and Skoog's semi-solid basal medium supplemented with several combinations of auxins and cytokinins. The greatest number of somatic embryos was obtained with a continuous exposure to 22.8 μM indoleacetic acid and 0.046 μM kinetin. The frequency of somatic embryogenesis from zygotic embryos depends on the cold conservation time of seeds. The embryos frequently germinated on the same medium. Further development of somatic embryos into plantlets was achieved on a medium devoid of growth regulators.
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Abbreviations
- MS:
-
Murashige and Skoog's medium
- IAA:
-
indole-3-acetic acid
- NAA:
-
α-naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IBA:
-
indole-3-butyric acid
- BAP:
-
6-benzylaminopurine
- RH:
-
relative humidity
References
Ammirato PV (1983) In: Evans DA, Sharp WR, Ammirato PV and Yamada Y (eds) Handbook of plant cell culture, Vol 1, Mac Millan Publishing Co, New York, pp 82–123
Beranger-Novat N, Dawidowicz-Grzegorzewska A (1992) Ann.Bot. 70: 93
Beranger-Novat N, Monin J, Singh CP (1976) CR. Soc. Biol. 171: 82–86
Carman JG, Jefferson NE, Campbell XF (1988) Plant Cell Tissue Organ Culture 12: 97–110
Chee RP, Cantlife DJ (1989) J. Plant Physiol. 135: 398–403
El Hadrami I, Carron MP, d'Auzac J (1991) Ann.Bot. 67: 511–515
Evans DA, Sharp WR, Flick E (1981) In: Thorpe TA (ed) Plant Tissue Culture. Methods and Application in Agriculture. Academic Press, New York, London. Toronto. Sydney, San Francisco, pp 45–113.
Geneve RL, Kester ST (1990) Plant Cell Tissue Organ Culture 22: 71–76
Hu CY, Sussex IM (1971) Phytomorphology 21: 103–107
Kochba J, Spiegel-Roy P, Neumann H, Saad S (1978) Z. Pflanzenphysiol. 89: 427–432
Lazzeri PA, Hildebrand DF, Collins GB (1987) Plant Cell Tissue Organ Culture 10: 197–208
Michler CH, Bauer EO (1991) Plant Science 77: 111–118
Mitrakos K, Alexaki A, Papadimitriou P (1992) J.Plant Physiol. 139: 269–273
Monin J (1966) C.R. Soc. Biol. 160: 2262–2264.
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497
Orinos TH, Mitrakos K (1991) Plant Cell Tissue Organ Culture 27: 183–188
Sommer HE, Brown CL (1980) For. Sci. 26: 257–260
Trigiano RN, Beaty RM, Graham ET (1988) Plant Cell Rpt. 7: 148–150
Weaver LA, Trigiano RN (1991) Plant Cell Rpt. 10: 183–186
Yeoman MN (1986) In: Withers LA, Alderson PG (eds) Plant Tissue Culture and its Agricultural Applications. Butterworth, Boston, Durban, Singapore, Sydney, Toronto, Wellington, pp 489–500
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Communicated by A.M. Boudet
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Bonneau, L., Beranger-Novat, N. & Monin, J. Somatic embryogenesis and plant regeneration in a woody species: the European Spindle Tree (Euonymus europaeus L.). Plant Cell Reports 13, 135–138 (1994). https://doi.org/10.1007/BF00239879
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DOI: https://doi.org/10.1007/BF00239879