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Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa L.)

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Abstract

Protoplasts were isolated from embryogenic cell suspensions obtained from mature seed derived embryogenic callus of the advanced Indica type rice breeding line IR72 available from IRRI (International Rice Research Institute), Manila. Culture of protoplasts with the agarose bead type method without nurse culture led to sustained proliferation of protoplast derived clones. A simple culture protocol was developed which stimulated embryogenic development. Germination of somatic embryos has so far produced 277 green plants from 6 independent experiments. 117 plants have been transferred to soil and are growing in the greenhouse. A few of them have already flowered and set seeds.

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Abbreviations

2,4-D, 2,4:

Dichlorophenoxyacetic acid

BAP:

Benzylaminopurine

CH:

Casein hydrolysate

ECS:

Embryogenie cell suspension

Kn:

Kinetin

NAA:

Napthaleneacetic acid; Media:

AA:

Muller and Grafe 1978

MS:

Murashige and Skoog 1962

N6:

Chu et al. 1975

R2:

Ohira et al. 1973

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Authors and Affiliations

  1. ETH-Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland

    Karabi Datta, Ingo Potrykus & Swapan K. Datta

Authors
  1. Karabi Datta
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  2. Ingo Potrykus
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  3. Swapan K. Datta
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Communicated by H. Lörz

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Datta, K., Potrykus, I. & Datta, S.K. Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa L.). Plant Cell Reports 11, 229–233 (1992). https://doi.org/10.1007/BF00235071

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  • DOI: https://doi.org/10.1007/BF00235071

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