Abstract
Efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar ‘Centennial’ on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. ‘Centennial’ expiants inoculated with Agrobacterium tumefaciens containing NPT II and GUS genes produced kanamycin-resistant calli from which buds were regenerated. Transformation and stable integration of transgenes was confirmed by GUS assay and DNA hybridization in kanamycin-resistant calli, and GUS assay in regenerated shoots.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- NAA:
-
1-naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS:
-
Murashige and Skoog (1962)
- GUS:
-
β-glucuronidase
- IAA:
-
indole-3-acetic acid
- NPT II:
-
neomycin phosphotransferase II
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Communicated by G. C. Phillips
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Ying, M., Dyer, W.E. & Bergman, J.W. Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. ‘Centennial’. Plant Cell Reports 11, 581–585 (1992). https://doi.org/10.1007/BF00233097
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DOI: https://doi.org/10.1007/BF00233097