Abstract
The possibility of obtaining haploid plants of chicory (Cichorium intybus L.) was investigated through intergeneric hybridization. Chicory plants (industrial chicory and Chioggia) were pollinated with pollen of Lactuca tatarica L. and Cicerbita alpina Walbr. Many achenes contained embryos which were rescued in vitro. Only a few embryos developed into plants which were then acclimatized in soil. Among them, three expressed a chicory phenorype and were haploid
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Abbreviations
- DAPI:
-
4,6-diamidinophenylindole
- DH:
-
doubled haploid
- DNA:
-
deoxyribonucleic acid
- GUS:
-
ß glucuronidase
- RFLP:
-
Restriction Fragment Length Polymorphism
- X-Gluc:
-
5-bromo-4-chloro-3-indolyl-glucuronide
- mtDNA:
-
mitochondrial DNA
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Doré, C., Prigent, J. & Desprez, B. In situ gynogenetic haploid plants of chicory (Cichorium intybus L.) after intergeneric hybridization with Cicerbita alpina Walbr.. Plant Cell Reports 15, 758–761 (1996). https://doi.org/10.1007/BF00232223
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DOI: https://doi.org/10.1007/BF00232223