Summary
Protoplasts with high embryogenic competence could be isolated from leaf-disk-derived embryos and embryoids of Vitis sp. cv. Seyval blanc. After a 4-week induction treatment in NN-69 medium supplemented with 4.0mg/l naphthoxyacetic acid (NOA) and 0.9mg/l thidiazuron (TDZ) and subsequent subcultivation in hormone-free medium, 38.5% of the developed microcalluses showed somatic embryogenesis. In contrast, only few formed somatic embryos after induction in CPW-13 medium with either 1.0mg/l 2,4-dichlorophenoxyacetic acid and 0.5mg/l benzylaminopurine treatment (13.8%) or NOA/TDZ treatment (1.4%). Up to 30% of these embryos germinated and about half of them regenerated into typical in vitro grapevines when transferred onto LS-medium in culture tubes.
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Abbreviations
- BAP:
-
benzylaminopurine
- BSA:
-
bovine serum albumin
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MES:
-
2-[N-morpholino]-ethanesulfonic acid
- NOA:
-
naphthoxyacetic acid
- PCR:
-
polymerase chain reaction
- PVP:
-
polyvinylpyrrolidone
- TDZ:
-
thidiazuron
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Communicated by I. Potrykus
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Reustle, G., Harst, M. & Alleweldt, G. Plant regeneration of grapevine (Vitis sp.) protoplasts isolated from embryogenic tissue. Plant Cell Reports 15, 238–241 (1995). https://doi.org/10.1007/BF00193727
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DOI: https://doi.org/10.1007/BF00193727