Summary
Penicillium occitanis strain Pol6, a mutant developed for hyperproduction of cellulase and pectinase enzymes was used for the study of extracellular pectinase production when pectins from different sources (apple and citrus) and with varying degree of esterification (DE) were used as inducers. Highly esterified citrus pectins were found to be suitable substrates for polygalacturonase, pectinase and pectin methyl esterase production, while low esterified citrus pectin favoured pectin lyase (PL) production. Apple pectins induced other hydrolytic enzymes (e.g., β-1,3-glucanase, β-glucosidase, β-galactosidase), in addition to pectolytic enzymes. Moreover, the combination of high and low esterified citrus pectins induced the production of a complete pectinase complex. The extent of degradation of the substrate and the affinity for PL decreased with decreasing DE irrespective of the source. There was no evidence of PL activity in this strain. No significant effect of cations (Ca++, Mn++, Na+) on PL activity was observed. However, EDTA (100 mm) inhibited 50% of the activity, when tested on highly esterified (rapid set citrus) pectin.
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References
Aguilar G, Huitron C (1986) Application of fed-batch cultures in the production of extracellular pectinases by Aspergillus sp. Enzyme Microb Technol 9:541–545
Albersheim P (1966) Pectin lyase from fungi. Methods Enzymol 8:628–631
Albersheim P, Neukom H, Deuel H (1960a) Formation of unsaturated dehydration products by a pectin degrading enzyme. Helv Chim Acta 43:1422–1426
Albersheim P, Neukom H, Deuel H (1960b) Splitting of pectin chain molecules in neutral solutions. Arch Biochem Biophys 90:46–51
Bateman DF (1966) Hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes of Fusarium solani f. phaseoli. Phytopathology 56:238–244
Beldman G, Rombouts FM, Voragen AGJ, Pilnik W (1984) Application of cellulase and pectinase from fungal origin for the liquefaction and saccharification of biomass. Enzyme Microb Technol 6:503–507
Bush DA, Codner RC (1968) The nature of macerating factor of Penicillium digitatum Saccardo. Phytochemistry 7:863–869
Durand H, Soucaille P, Tiraby G (1984) Comparative study of cellulases and hemicellulases from four fungi: mesophiles Trichoderma reesei and Penicillium sp. and thermophiles Thielavia terrestris and Sporotrichum cellulophilum. Enzyme Microb Technol 6:175–180
Edstrom RD, Phaff HJ (1964a) Purification and certain properties of pectin trans-eliminase from Aspergillus fonsecaeus. J Biol Chem 239:2403–2408
Edstrom RD, Phaff HJ (1964b) Elimination cleavage of pectin and of oligogalacturonide methyl esters by pectin trans-eliminase. J Biol Chem 239:2409–2415
Ellouz S, Durand H, Tiraby G (1987) Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography. J Chromatogr 396:307–317
Fogarty WM, Kelly CT (1983) Pectic enzymes. In: Fogarty WM (ed) Microbial enzymes and biotechnology. Applied Science Publishers, London, pp 131–182
Jain S, Tiraby G (1987) Separation and characterization of the cellulolytic components of a thermophilic fungus, Talaromyces sp. CL 240. In: Grassi G, Delmon B, Molle JF, Zibetta H (eds) Proceedings of the 4th EC Conference on Biomass for Energy and Industry. Elsevier, London, pp 355–359
Jain S, Sarriche M, Durand H, Tiraby G (1990) Production of polysaccharidases by a cellulase-pectinase hyperproducing mutant (Pol6) of Penicillium occitanis. Enzyme Microb Technol 12:691–696
Kalb VF, Bernlohr RW (1977) A new spectrophotometric assay for protein in cell extracts. Anal Biochem 82:362–371
Lobanok AG, Mikhailova RV, Sapunova LI (1977) Kinetics of the constitutive synthesis of polymethylgalacturonase by Penicillium digitatum in relation to the carbon source and pH. Mikrobiologiya 46:920–925
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275
Maldonado MC, Navarro A, Callieri DAS (1986) Production of pecti nases by Aspergillus sp. using differently pretreated lemon peel as the carbon source. Biotechnol Lett 8:501–504
Mandels M, Weber J (1969) The production of cellulases. Adv Chem Ser 95:391–414
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Rexova-Benkova L, Markovic O (1976) Pectic enzymes. Adv Carbohydr Chem Biochem 33:323–385
Saval S, Huitron C (1983) Microbial pectinases from Henequen pulp. Dev Ind Microbiol 24:547–551
Sherwood RT (1966) Pectin lyase and polygalacturonase production by Rhizoctonia solani and other fungi. Phytopathology 56:279–286
Spalding DH, Abdul-Baki AA (1973) In vitro and in vivo production of pectin lyase by Penicillium expansum. Phytopathology 63:231–235
Vazquez C, Martinez MJ, Lahoz R, Reyes F (1986) Effect of calcium and other metal ions on pectic activities from autolysed cultures of Alternaria alternata. FEMS Microbiol Lett 37:227–230
Whitaker JR (1984) Pectic substances, pectic enzymes and haze formation in fruit juices. Enzyme Microb Technol 6:341–349
Zimmerman RE (1978) A rapid assay for pectinesterase activity which can be used as a prescreen for pectinesterase inhibitors. Anal Biochem 85:219–223
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Jain, S., Durand, H. & Tiraby, G. Production of extracellular pectinase enzymes by a mutant (Pol6) of Penicillium occitanis . Appl Microbiol Biotechnol 34, 308–312 (1990). https://doi.org/10.1007/BF00170048
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DOI: https://doi.org/10.1007/BF00170048