Abstract
A new member of theLea B19 gene family from barley, termed B19.1b, has been isolated and characterized. The coding region of B19.1b is highly similar to the other members of the B19 family (Espelundet al., Plant J 2 (1992) 241–252) and contains only one copy of the hydrophilic sequence found as a repeat in two other B19 genes. Like the other B19 genes, B19.1b is only expressed in embryos. The transcript appears during development at 25 days after anthesis and remains at a high level throughout embryogenesis. In immature embryos the B19.1b mRNA can be induced by abscisic acid, salt and mannitol.
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References
Baker J, Steele C, Dure LIII: Sequence and characterization of 6Lea proteins and their genes from cotton. Plant Mol Biol 11: 277–291 (1988).
Bäumlein H, Nagy I, Villarroel R, Inzé D, Wobus U:Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within the legumin box is essential for tissue-specific expression of a legumin gene. Plant J 2: 233–239 (1992).
Chen Z-L, Pan N-S, Beachy RN: A DNA sequence element that confers seed-specific enhancement to a constitutive promoter. EMBO J 7: 297–302 (1988).
Close TJ, Kortt AA, Chandler PM: A cDNA-based comparison of dehydration-induced protein (dehydrins) in barley and corn. Plant Mol Biol 13: 95–108 (1989).
Espelund M, Jakobsen KS. Cloning and direct sequencing of plant promoters using primer-adapter mediated PCR on DNA coupled to a magnetic solid phase. Bio-Techniques 13: 74–81 (1992).
Espelund M, Stacy RAP, Jakobsen KS: A simple method for generating single-stranded DNA probes labeled to high activities. Nucl Acids Res 18: 6157–6158 (1990).
Espelund M, Sæbøe-Larssen S, Hughes DW, Galau GA, Larsen F, Jakobsen KS: Late embrygenesis-abundant genes encoding proteins with different numbers of hydrophilic repeats are regulated differentially by abscisic acid an osmotic stress. Plant J 2: 241–252 (1992).
Futers TS, Onde S, Turet M, Cuming AC: Sequence analysis of two tandemly linked Em genes from wheat. Plant Mol Biol 23: 1067–1072 (1993).
Galau GA, Bijaisoradat N, Hughes DW: Accumulation kinetics of cotton late embryogenesis-abundant mRNAs and storage protein mRNAs: coordinate regulation during embryogenesis and the role of abscisic acid. Devel Biol 123: 198–212 (1987).
Gaubier P, Raynal M, Hull G, Huestis GM, Grellet F, Arenas C, Pagès M, Delseny M. Two differentEm-like genes are expressed inArabidopsis thaliana seeds during maturation. Mol Gen Genet 238: 409–418 (1993).
Guiltinan MJ, Marcotte WRJr, Quatrano RS: A plant leucine zipper protein that recognizes an abscisic acid response element. Science 250: 267–271 (1990).
Hattori T, Vasil V, Rosenkrans L, Hannah LC, McCarty DR, Vasil IK. TheViviparous-1 gene and abscisic acid activate theC1 regulatory gene for anthocyanin biosynthesis during seed maturation in maize. Genes Devel 6: 609–618 (1992).
Hughes DW, Galau GA: Developmental and environmental induction ofLea andLeaA mRNAs and the post-abscission program during embryo culture. Plant Cell 3: 605–618 (1991).
Hultman T, Ståhl S, Hornes E, Uhlèn M: Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucl Acids Res 17: 4937–4946 (1989).
Jakobsen KS, Breivold E, Hornes E: Purification of mRNA directly from crude plant tissue in 15 minutes using magnetic oligo dT microspheres. Nucl Acids Res 18: 3669 (1990).
Jakobsen KS, Haugen M, Sæbøe-Larssen S, Hollung K, Espelund M, Hornes E: Direct mRNA isolation using magnetic oligo dT beads: a protocol for all types of cell cultures, animal and plant tissues. In: Uhlén M, Hornes E, Olsvik Ø (eds) Advances in Biomagnetic Separation. Eaton Publishers, Natick, pp. 61–71 (1994).
Jakobsen KS, Hughes DW, Galau GA: Simultaneus induction of postabscission and germination mRNAs in cultured dicot embryos. Planta 192: 384–394 (1994).
Kawagoe Y, Murai N: Four distinct nuclear proteins recognizein vitro the proximal promoter of the bean seed storage proteinβ-phaseolin gene conferring spatial and temporal control. Plant J 2: 927–936 (1992).
Litts JC, Colwell GW, Chakerian RL, Quatrano RS: The nucleotide sequence of a cDNA clone encoding the wheat Em protein. Nucl Acids Res 15: 3607–3618 (1987).
Litts JC, Colwell GW, Chakerian RL, Quatrano RS: Sequence analysis of a functional member of the Em gene family from wheat. DNA Sequence 1: 263–274 (1991).
Litts JC, Erdman MB, Huang N, Karrer EE, Noueiry A, Quatrano RS, Rodriguez RL: Nucleotide sequence of the rice (Oryza sativa) Em protein gene (Emp1). Plant Mol Biol 19: 335–337 (1992).
Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).
Williams ME, Foster R, Chua N-H: Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding. Plant Cell 4: 485–496 (1992).
Wurtele ES, Wang H, Durgerian S, Nikolau BJ, Uhlrich TH: Characterization of a gene that is expressed early in somatic embryogenesis ofDaucus carota. Plant Physiol 102: 303–312 (1993).
Yamaguchi-Shinozaki K, Mundy J, Chua N-H: Four tightly linkedrab genes are differentially expressed in rice. Plant Mol Biol 14: 29–39 (1989).
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Hollung, K., Espelund, M. & Jakobsen, K.S. AnotherLea B19 gene (Group1Lea) from barley containing a single 20 amino acid hydrophilic motif. Plant Mol Biol 25, 559–564 (1994). https://doi.org/10.1007/BF00043884
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DOI: https://doi.org/10.1007/BF00043884