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A programmable micropropagation apparatus using cycled liquid medium

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Abstract

An apparatus was developed that featured programmable application of liquid medium to plant cultures for micropropagation. Computer control capabilities included liquid medium introduction and medium depth within four culture vessels, medium application and removal on an assigned schedule, schedule adjustment during a culture period and medium replacement. The medium level was controlled using an accurate custom level-sensing technique consisting of thermistors and float switches. Seven-liter polycarbonate containers were modified and used as the culture vessels. Maintaining sterility was a key constraint in the development of the plant tissue culture apparatus.

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References

  • Aitken-Christie J & Davies H (1988) Development of a semi-automated micropropagation system. Acta Hort. 230: 81–87

    Google Scholar 

  • Florkowski WJ, Lindstrom O, Robacker C & Simonton W (1988) Biological, Technical, and Economic Aspects of Micropropagation. Research Report #556 of the Georgia Agricultural Experiment Stations, University of Georgia

  • Hamilton R, Pederson H & Chin CK (1985) Plant tissue culture on membrane rafts. BioTechniques 3: 96

    Google Scholar 

  • Harrell R & Simonton W (1986) Automation opportunities in plant tissue culture. Amer. Soc. Agr. Engineers, Technical Paper No 86-1596

  • Hussey G (1986) Problems and prospects in the in vitro propagation of herbaceous plants. In: Withers LA & Alderson PG (Eds) Plant Tissue Culture and Its Agricultural Applications (pp 69–84). Butterworths, Boston, MA

    Google Scholar 

  • Kozai T (1988) High technology in protected cultivation-from environmental control engineering point of view. In: Kozai T (Ed) Horticulture in High Technology Era (pp 1–43). Tokyo, Japan

  • Kozai T, Fukiwara K & Watanbe I (1986) Fundamental studies on environments in plant tissue culture vessels. J. Agr. Met. 42(2): 119–127

    Google Scholar 

  • Krueger S (1990) Micropropagation of Amelanchier x grandiflora ‘Princess Diana’ in a semi-automated culture system. Master's Thesis, University of Georgia (p 53)

  • Levin R, Gaba V, Tal B, Hirsch S, DeNola D & Vasil I (1988) Automated plant tissue culture for mass propagation. Bio/Technology 6: 1035–1040

    Google Scholar 

  • Maene L & Debergh P (1985) Liquid medium additions to established tissue cultures to improve elongation and rooting in vivo. Plant Cell Tiss. Org. Cult. 5: 23–33

    Google Scholar 

  • Miller L & Murashige T (1976) Tissue culture propagation of tropical foliage plants. In Vitro 12: 797–813

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Reuther G (1985) Principles and application of the micropropagation of ornamental plants. In: Schäfer-Menuhr A (Ed) In Vitro Techniques: Propagation and Long-Term Storage (pp 1–14). Martinus Nijhoff, Dordrecht, Netherlands

    Google Scholar 

  • Simonton W & Robacker C (1988) Alternative system for micropropagation. Amer. Soc. Agr. Engineers, Technical Paper No. 88-1028

  • Sluis CJ & Walker KA (1985) Commercialization of plant tissue culture. Intl. Assoc. Plant Tiss. Cult. Newsl. No 47: 2–12

  • Stevenson JH, Harris RE & Monette PL (1982) A comparison of liquid and semi-solid culture media for the in vitro proliferation of Nicotiana tabacum cv. Xanthi-nc. Plant Propagator 28(3): 12–14

    Google Scholar 

  • Taylor ME & Knauss JF (1978) Tissue culture multiplication and subsequent handling of known pathogen-free Dieffenbachia maculata cv. Perfection. Proc. Fla. State Hort. Soc. 91: 233–235

    Google Scholar 

  • Tisserat B & Vandercook CE (1985) Development of an automated plant culture system. Plant Cell Tiss. Org. Cult. 5: 107–117

    Google Scholar 

  • Tisserat B & Vandercook CE (1986) Computerized long-term tissue culture for orchids. Amer. Orchid. Soc. Bull. 55(1): 35–42

    Google Scholar 

  • Walker PN, Heuser CW & Heinemann PH (1988) Micropropagation: Studies of gaseous environments. Acta Hort. 230: 145–151

    Google Scholar 

  • Weathers PJ, Cheetham RD & Giles KL (1988) Dramatic increases in shoot number and lengths for Musa, Cordyline and Nephrylepsis [sic] using nutrient mists. Acta Hort. 230: 39–44

    Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Engineering, Georgia Experiment Station, 30223, Griffin, GA, USA

    Ward Simonton

  2. Department of Horticulture, Georgia Experiment Station, 30223, Griffin, GA, USA

    Carol Robacker & Scott Krueger

Authors
  1. Ward Simonton
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  2. Carol Robacker
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  3. Scott Krueger
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Simonton, W., Robacker, C. & Krueger, S. A programmable micropropagation apparatus using cycled liquid medium. Plant Cell Tiss Organ Cult 27, 211–218 (1991). https://doi.org/10.1007/BF00041292

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  • DOI: https://doi.org/10.1007/BF00041292

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