Abstract
Cultivating Vitis vinifera cell suspensions in a production medium which is characterized by high sucrose and low nitrate concentrations (132 mM and 6.25 mM respectively) repressed growth but enhanced the intracellular accumulation of anthocyanins, especially peonidin 3-glucoside. Increasing the ammonium concentration of the production medium from 2 to 8–16 mM increased growth and decreased the accumulation of anthocyanins and peonidin 3-glucoside specifically. Instead, peonidin 3-p-coumaroylglucoside accumulated. At 24 mM ammonium concentration, growth was inhibited and accumulation of peonidin 3-p-coumaroylglucoside was significant (p<0.05) and represented 42% of total anthocyanins after 12 days of culture compared with 19% in the production medium with 2 mM ammonium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amino SI & Tazawa M (1988) Uptake and utilization of sugars in cultured rice cells. Plant Cell Physiol. 29: 483–488
Cormier F, Crevier H & Do CB (1990) Effects of sucrose concentration on the accumulation of anthocyanins in grape (Vitis vinifera L.) cell suspension. Can. J. Bot. 68: 1822–1826
Do CB & Cormier F (1990) Accumulation of anthocyanins enhanced by a high osmotic potential in grape (Vitis vinifera L.) cell suspension. Plant Cell Rep. 9: 143–146
Do CB & Cormier F (1991) Accumulation of peonidin 3-glucoside enhanced by osmotic stress in grape (Vitis vinifera L.) cell suspension. Plant Cell Tiss. Org. Cult. 24: 49–54
Do CB & Cormier F (1991) Effects of low nitrate and high sugar concentrations on anthocyanin content and composition of grape (Vitis vinifera L.) cell suspension. Plant Cell Rep. 9: 500–504
Dougall DK & Frazier GC (1989) Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells. Plant Cell Tiss. Org. Cult. 18: 95–104
Francis FJ (1982) Analysis of anthocyanins. In: Markakis P (Ed) Anthocyanin as Food Color (pp 181–208). Academic Press, London
Francis FJ (1989) Food colorant: Anthocyanins. In: Critical Review in Food Science and Nutrition, Vol. 28(4) (pp 273–314). CRC Press
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Gamborg OL & Shyluk JP (1970) The culture of plant cells with ammonium salts as the sole nitrogen source. Plant Physiol. 45: 598–600
Ikeda T, Matsumoto T & Noguchi M (1977) Effects of inorganic nitrogen sources and physical factors on the formation of ubiquinone by tobacco plant cells in suspension culture. Agric. Biol. Chem. 41 (7): 1197–1201
Lofty S, Fleuriet A, Ramos T & Macheix JJ (1989) Biosynthesis of phenolic compounds in Vitis vinifera cell suspension cultures: Study on hydroxycinnamoyl CoA: ligase. Plant Cell Rep. 8: 93–96
Masuda H, Takahashi T & Sugawara S (1988) Acid and alkaline invertases in suspension cultures of sugar beet cells. Plant Physiol. 86: 312–317
Nelson WL (1944) A photometric adaptation of the Symogyi method for the determination of glucose. J. Bio. Chem. 153: 375–380
Nakagawa K, Konagai A, Fukui H & Tabata M (1984) Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Rep. 3: 254–257
Sargent PA & King J (1974) Investigations of growth promoting factors in conditioned soybean root cells and in the liquid medium in which they grow: ammonium, glutamine and amino acids. Can. J. Bot. 52: 1747–1755
SAS (1987) SAS/STAT. Guide for Personal Computer. Version 6. SAS Institute Inc. NC. USA
Schmitz U & Lorz H (1990) Nutrient uptake in suspension cultures of Graminae. II-Suspension cultures of rice (Oryza sativa L.). Plant Science 66: 95–111
Tal B & Goldberg I (1982) Growth and diosgenin production by Dioscorea deltoidea cells in batch and continuous cultures. Planta Med. 44: 107–110
Tal B, Gressel J & Goldberg I (1982) The effect of medium constituents on growth and diogenin production by Dioscorea deltoidea cells grown in batch culture. Planta Med. 44: 111–115
Teusch M, Forkmann G & Seyffert W (1987) Genetic control of hydroxycinnamoyl CoA: anthocyanidin 3-glycoside-hydroxycinnamoyl transferase from petals of Matthiola incana. Phytochem. 26: 991–994
Van Calsteren MR, Cormier F, Do CB & Laing RR (1991) 1H and 13C NMR assignments of the major anthocyanins from Vitis vinifera cell suspension culture. Spectros. Int. J. (in press)
Weatherburn MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 39 (8): 971–974
Westcott RJ & Henshaw GG (1976) Phenolic synthesis and phenylalanine ammonia lyase activity in suspension cultures of Acer pseudoplatanus L. Planta 131: 67–73
Yamakawa T, Kato S, Ishida K, Kodama T & Minoda Y (1983) Production of anthocyanins by Vitis cells in suspension culture. Agri. Biol. Chem. 47: 2185–2191
Yamamoto Y, Kinoshita Y, Watanabe S & Yamada Y (1989) Anthocyanin production in suspension cultures of high producing cells of Euphorbia millii. Agric. Biol. Chem. 53(2): 417–423
Zapalis C & Francis FJ (1965) Cranberry anthocyanins. J. Food Sci. 30: 396–399
Author information
Authors and Affiliations
Additional information
Contribution Number 217.
Rights and permissions
About this article
Cite this article
Do, C.B., Cormier, F. Effects of high ammonium concentrations on growth and anthocyanin formation in grape (Vitis vinifera L.) cell suspension cultured in a production medium. Plant Cell Tiss Organ Cult 27, 169–174 (1991). https://doi.org/10.1007/BF00041286
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00041286